Plasmid encoded lacticin 481 production in Lactococcus lactis subsp lactis CM41

被引：0
作者：
Tükel, Q
Akçelik, M
机构：
[1] Ankara Univ, Fac Agr, Dept Food Engn, TR-06110 Ankara, Turkey
[2] Ankara Univ, Fac Sci, Dept Biol, TR-06100 Ankara, Turkey
来源：
MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL | 2004年 / 59卷 / 11-12期
关键词：
Lactococcus lactis (production of bacteriocin);
D O I：
暂无
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Twenty L. lactis subsp. lactis strains, isolated from raw milk and whey samples, were screened for their antimicrobial activity. One of the strains was selected for its broad activity spectrum of inhibition against various food pathogens, including Listeria and Clostridium strains. Proteolytic enzymes confirmed the proteinaceous nature of the substance. Bacteriocin was purified by ammonium sulphate, ion exchange and reverse phase chromotography. Amino acid sequence showed strong homology with lacticin 481. This result was confirmed by PCR amplification and DNA sequence analysis. Lacticin 481 was encoded by a 52.0 kb conjugative plasmid in L. lactis subsp. lactis CM41.
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页码：601 / 604
页数：4
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