Yeast-extract improved biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L.

Lignans and neolignans are important biologically active ingredients (BAIs) biosynthesized by Linum usitatissimum. These BAIs have multi-dimensional effects against cancer, diabetes and cardio vascular diseases. In this study, yeast extract (YE) was employed as an elicitor to evaluate its effects on dynamics of biomass, BAIs and antioxidant activities in L. usitatissimum cell cultures. During preliminary experiments, flax cultures were grown on different concentrations of YE (0-1000mg/L), and 200mg/L YE was found to be optimum to enhance several biochemical parameters in these cell cultures. A two-fold increase in fresh (FW) and dry weight (DW) over the control was observed in cultures grown on MS medium supplemented with 200mg/L YE. Similarly, total phenolic (TPC; 16mg/g DW) and flavonoids content (TFC; 5.1mg/g DW) were also positively affected by YE (200mg/L). Stimulatory effects of YE on biosynthesis of lignans and neolignans was also noted. Thus, 200mg/L of YE enhanced biosynthesis of secoisolariciresinol diglucoside (SDG; 3.36-fold or 10.1mg/g DW), lariciresinol diglucoside (LDG; 1.3-fold or 11.0mg/g DW) and dehydrodiconiferyl alcohol glucoside (DCG; 4.26-fold or 21.3mg/g DW) in L. usitatissimum cell cultures with respect to controls. This elicitation strategy could be scaled up for production of commercially feasible levels of these precious metabolites by cell cultures of Linum.