Simultaneous Detection of Multiple Single-Nucleotide Polymorphisms by a Simple Membrane Chip

被引:0
作者
Yang, Yu-Hsiang [2 ]
Li, Ruei-Nian [1 ]
Tzou, Shey-Cherng [3 ]
Wang, Jaw-Yuan [4 ]
Lee, Hsin-Pai [5 ]
Wang, Hsien-Chung [5 ]
Chen, Fang-Ming [4 ]
Wang, Yung-Hsin [2 ]
Hsieh, Ming-Chia [2 ]
Huang, Ming-Yii [6 ]
Tseng, Wei-Lung [7 ,8 ]
Lin, Shiu-Ru [3 ,9 ]
Cheng, Tian-Lu [1 ,8 ]
机构
[1] Kaohsiung Med Univ, Dept Biomed Sci & Environm Biol, Kaohsiung 80707, Taiwan
[2] Kaohsiung Med Univ, Grad Inst Med Genet, Kaohsiung 80707, Taiwan
[3] Fooyin Univ, Sch Med & Hlth, Kaohsiung, Taiwan
[4] Kaohsiung Med Univ Hosp, Ctr Canc, Dept Surg, Kaohsiung, Taiwan
[5] Pingtung Christian Hosp, Dept Orthoped, Pingtung, Taiwan
[6] Kaohsiung Med Univ, Dept Radiat Oncol, Kaohsiung, Taiwan
[7] Natl Sun Yat Sen Univ, Dept Chem, Kaohsiung 80424, Taiwan
[8] Kaohsiung Med Univ, Joint Res Ctr, Natl Sun Yat Sen Univ, Kaohsiung 80424, Taiwan
[9] Fooyin Univ Hosp, Dept Med Res, Pingtung, Taiwan
关键词
CORONARY-HEART-DISEASE; DENSITY-LIPOPROTEIN CHOLESTEROL; CARDIOVASCULAR-DISEASE; ANGIOTENSINOGEN GENE; M235T POLYMORPHISM; APOLIPOPROTEIN-E; RISK; VARIANT; AMPLIFICATION; METAANALYSIS;
D O I
10.1089/gtmb.2010.0032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Technologies that screen multiple single-nucleotide polymorphisms (SNPs) could be very valuable in predicting patients' susceptibilities to diseases or responses to therapeutic interventions. In this study, we developed a chip that can accurately detect four SNPs at same time. This chip is cost-effective and user-friendly because it uses a detection protocol analogous to dot blotting and does not require sophisticated instruments. To establish this chip, we designed and blotted onto a nylon membrane SNP-specific oligonucleotide probes for human angiotensinogen, cholesteryl ester transfer protein, and apolipoprotein E. This chip detected the corresponding SNPs harbored within the angiotensinogen, cholesteryl ester transfer protein, and apolipoprotein E sequences from 20 donors. Importantly, the SNPs detected by our chip matched exactly with the direct sequencing results, thereby highlighting the accuracy of this chip. In conclusion, our chip is a robust tool for multiple SNP screening and holds the potential to future refinement in detecting diseases-associating genes in patients.
引用
收藏
页码:653 / 659
页数:7
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