Characterization of a strain of Apple stem grooving virus in Actinidia chinensis from China

被引:85
作者
Clover, GRG
Pearson, MN
Elliott, DR
Tang, Z
Smales, TE
Alexander, BJR
机构
[1] Minist Agr & Forestry, Natl Plant Pest Reference Lab, Auckland, New Zealand
[2] Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand
关键词
Apple stem grooving virus; Capillovirus; Chinese gooseberry; DAS-ELISA; immunocapture RT-PCR; kiwifruit;
D O I
10.1046/j.1365-3059.2003.00857.x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A new strain of Apple stem grooving virus (ASGV) has been identified in Actinidia chinensis imported from China. The leaves of these plants exhibited a variety of symptoms including interveinal mottling, chlorotic mosaics and ringspots. Capillovirus-like particles were observed under the electron microscope, and the virus could be mechanically transmitted to a range of herbaceous indicators. The virus was detected using ELISA with antisera raised against ASGV. Sequencing of the virus revealed that it had more than 95% amino acid identity with ASGV in the putative coat and movement proteins. From the morphological, transmission, serological and molecular evidence, it was concluded that the virus is a strain of ASGV. It is not known how this strain of ASGV is transmitted, other than by grafting, nor is it known what effect the virus has on the growth of infected vines. The Actinidia -infecting strain of ASGV does not occur in New Zealand, and infected plants will not be released from quarantine. The detection methods used during the research will assist quarantine and the safe movement of breeding material.
引用
收藏
页码:371 / 378
页数:8
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