Characteristics of High-Molecular-Weight Hyaluronic Acid as a Brain-Derived Neurotrophic Factor Scaffold in Periodontal Tissue Regeneration

被引：0

作者：

Takeda, Katsuhiro ^{[1
]}

Sakai, Noriyuki ^{[1
]}

Shiba, Hideki ^{[1
]}

Nagahara, Takayoshi ^{[1
]}

Fujita, Tsuyoshi ^{[1
]}

Kajiya, Mikihito ^{[1
]}

Iwata, Tomoyuki ^{[1
]}

Matsuda, Shinji ^{[1
]}

Kawahara, Kazuko ^{[1
]}

Kawaguchi, Hiroyuki ^{[1
]}

Kurihara, Hidemi ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Dept Periodontal Med, Div Frontier Med Sci,Minami Ku, Hiroshima 7348553, Japan

来源：

TISSUE ENGINEERING PART A | 2011年 / 17卷 / 7-8期

基金：

日本学术振兴会;

关键词：

IN-VITRO; MATRIX PROTEINS; LIGAMENT CELLS; EXPRESSION; CD44; FIBROBLASTS; PROLIFERATION; RECEPTOR; GROWTH; REPAIR;

D O I：

10.1089/ten.tea.2010.0070

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, a scaffold that does not contain unknown ingredients is preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) is safe and inexpensive, we evaluated the efficacy of HMW-HA as a BDNF scaffold. CD44, a major receptor of HA, was expressed in cultures of human periodontal ligament cells, and HMW-HA promoted the adhesion and proliferation of human periodontal ligament cells, although it did not influence the mRNA expression of bone (cementum)-related proteins. The in vitro release kinetics of BDNF from HMW-HA showed that BDNF release was sustained for 14 days. Subsequently, we examined the effect of BDNF/HMW-HA complex on periodontal tissue regeneration in dogs. A greater volume of newly formed alveolar bone and a longer newly formed cementum were observed in the BDNF/HMW-HA group than in the HMW-HA group, suggesting that HMW-HA assists the regenerative capacity of BDNF, although HMW-HA itself does not enhance periodontal tissue regeneration. Neither the poly (lactic-co-glycolic acid) group nor the BDNF/poly (lactic-co-glycolic acid) group enhanced periodontal tissue regeneration. In conclusion, HMW-HA is an adequate scaffold for the clinical application of BDNF.

引用

页码：955 / 967

页数：13

共 49 条

[1] CD44 IS THE PRINCIPAL CELL-SURFACE RECEPTOR FOR HYALURONATE [J].