The Role of OmpC and OmpF in Acidic Resistance in Escherichia coli

被引：28

作者：

Bekhit, Amany ^{[1
]}

Fukamachi, Toshihiko ^{[1
]}

Saito, Hiromi ^{[1
]}

Kobayashi, Hiroshi ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Pharmaceut Sci, Chiba 2608675, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2011年 / 34卷 / 03期

关键词：

acidic resistance; OmpC; OmpF; Escherichia coli; LYSINE DECARBOXYLASE GENES; MEMBRANE PORIN PROTEINS; OUTER-MEMBRANE; INDUCIBLE ARGININE; GLUTAMATE; SURVIVAL; K-12; EXPRESSION; STRESS;

D O I：

10.1248/bpb.34.330

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The roles of OmpC and OmpF in acidic resistance (AR) were examined. When ompC and ompF were deleted, AR was decreased. The decreased level of AR seen in the mutant that was deficient in ompC and ompF was elevated by the addition of glutamate, but not by the addition of arginine or lysine. The expression levels of adiA and cadB were diminished by the deletion of ompC and ompF, and the conversion of arginine to agmatine and lysine to cadaverine by intact cells were reduced in the mutant. The expression of gadA/gadB was not affected by the deletion of ompC and ompF. These results suggest that the transport of arginine, lysine, and their decarboxylated products through OmpC and/or OmpF is essential for the survival of Escherichia coli cells under extremely acidic conditions.

引用

页码：330 / 334

页数：5

共 33 条

[1] Arginine and lysine decarboxylases and the Acid Tolerance Response of Salmonella Typhimurium [J].

Alvarez-Ordonez, Avelino ;

Fernandez, Ana ;

Bernardo, Ana ;

Lopez, Mercedes .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2010, 136 (03) :278-282

[2] CONSTRUCTION OF LAC FUSIONS TO THE INDUCIBLE ARGININE AND LYSINE DECARBOXYLASE GENES OF ESCHERICHIA-COLI-K12 [J].

AUGER, EA ;

REDDING, KE ;

PLUMB, T ;

CHILDS, LC ;

MENG, SY ;

BENNETT, GN .

MOLECULAR MICROBIOLOGY, 1989, 3 (05) :609-620

[3] OmpR regulates the stationary-phase acid tolerance response of Salmonella enterica serovar Typhimurium [J].

Bang, IS ;

Kim, BH ;

Foster, JW ;

Park, YK .

JOURNAL OF BACTERIOLOGY, 2000, 182 (08) :2245-2252

[4] Escherichia coli O157: H7 glutamate- and arginine-dependent acid-resistance systems protect against oxidative stress during extreme acid challenge [J].

Bearson, Bradley L. ;

Lee, In Soo ;

Casey, Thomas A. .

MICROBIOLOGY-SGM, 2009, 155 :805-812

[5] Characterization of enterohemorrhagic Escherichia coli strains based on acid resistance phenotypes [J].

Bhagwat, AA ;

Chan, L ;

Han, R ;

Tan, J ;

Kothary, M ;

Jean-Gilles, J ;

Tall, BD .

INFECTION AND IMMUNITY, 2005, 73 (08) :4993-5003

[6] Interference of the CadC regulator in the arginine-dependent acid resistance system of Shigella and enteroinvasive E. coli [J].

Casalino, Mariassunta ;

Prosseda, Gianni ;

Barbagallo, Marialuisa ;

Iacobino, Angelo ;

Ceccarini, Paolo ;

Latella, Maria Carmela ;

Nicoletti, Mauro ;

Colonna, Bianca .

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 300 (05) :289-295

[7] Control of acid resistance in Escherichia coli [J].

Castanie-Cornet, MP ;

Penfound, TA ;

Smith, D ;

Elliott, JF ;

Foster, JW .

JOURNAL OF BACTERIOLOGY, 1999, 181 (11) :3525-3535

[8] Survival of nonspecific porin-deficient mutants of Escherichia coli in black sea water [J].

Darcan, C ;

Özkanca, R ;

Flint, KP .

LETTERS IN APPLIED MICROBIOLOGY, 2003, 37 (05) :380-385

[9] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].

Datsenko, KA ;

Wanner, BL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645

[10] When protons attack: microbial strategies of acid adaptation [J].

Foster, JW .

CURRENT OPINION IN MICROBIOLOGY, 1999, 2 (02) :170-174

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