Development of efficient method for purified recombinant bovine granulocyte-macrophage colony-stimulating factor production with baculovirus-silkworm gene expression system

被引：6

作者：

Nagaya, Hidekazu

Kanaya, Toshimichi

Tobita, Yoneko

Yokomizo, Yuichi

Inumaru, Shigeki

Onodera, Takashi

机构：

[1] Katakura Ind Co Ltd, Sayama, Saitama 3501332, Japan

[2] Kitayama Labes Co Ltd, Nagano 3994431, Japan

[3] Natl Inst Anim Hlth, Tsukuba, Ibaraki 3050856, Japan

[4] Univ Tokyo, Dept Mol Immunol, Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 01期

关键词：

baculovirus; bovine; GM-CSF; purification; silkworm;

D O I：

10.1007/s10529-007-9506-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant bovine granulocyte-macrophage colony-stimulating factor (rboGM-CSF) was produced by the baculovirus-silkworm expression system. It was purified to 98% by (NH4)(2)SO4, followed by a three-step column chromatography with silica gel, ion exchange resin and a metal chelate column. The specific activity of purified rboGM-CSF was 1.6-6.3 10(6) ED50 mg(-1). By this method, the specific activity was raised 160-fold and 21% of the expressed rboGM-CSF was recovered.

引用

页码：41 / 45

页数：5