Genistein in the control of breast cancer cell growth: insights into the mechanism of action in vitro

被引：101

作者：

Fioravanti, L

Cappelletti, V

Miodini, P

Ronchi, E

Brivio, M

Di Fronzo, G

机构：

[1] Ist Nazl Studio & Cura Tumori, I-20133 Milan, Italy

[2] CNR, Ctr Res Cell Pathol, I-20133 Milan, Italy

来源：

CANCER LETTERS | 1998年 / 130卷 / 1-2期

关键词：

breast cancer; cell cycle; estradiol; genistein; growth factors; tyrosine kinase inhibitors;

D O I：

10.1016/S0304-3835(98)00130-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Genistein significantly inhibited cell growth (IC50 around 10 mu M) of MCF-7, MDAMB-231 and HBL-100 cell lines, but not of skin-derived fibroblasts and counteracted the growth-stimulatory effects exerted by estradiol and growth factors. It abolished the paracrine stimulation observed in MCF-7 cells in co-culture with MDAMB-231 or fibroblasts. Genistein-treated cells accumulated in the S and G(2)/M phases of the cell cycle and underwent apoptosis. Genistein decreased tyrosine phosphorylation induced upon treatment with transforming growth factor-alpha. Finally, genistein bound the estrogen receptor (ER) (relative affinity constant K-d = 4 nM), induced pS2 and cathepsin-D transcription and increased nuclear ER levels. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：143 / 152

页数：10

共 31 条

[1] WESTERN DIET AND WESTERN DISEASES - SOME HORMONAL AND BIOCHEMICAL-MECHANISMS AND ASSOCIATIONS [J].