Unnatural amino acid mutagenesis in mapping ion channel function

被引：82
作者：
Beene, DL [1 ]
Dougherty, DA
Lester, HA
机构：
[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
[2] CALTECH, Div Biol, Pasadena, CA 91125 USA
来源：
CURRENT OPINION IN NEUROBIOLOGY | 2003年 / 13卷 / 03期
关键词：
D O I：
10.1016/S0959-4388(03)00068-0
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure-function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing experiments on ion channels.
引用
收藏
页码：264 / 270
页数：7
相关论文
共 32 条
[1]   Fluorescence resonance energy transfer between unnatural amino acids in a structurally modified dihydrofolate reductase [J].
Anderson, RD ;
Zhou, J ;
Hecht, SM .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (33) :9674-9675
[2]   Cation-π interactions in ligand recognition by serotonergic (5-HT3A) and nicotinic acetylcholine receptors:: The anomalous binding properties of nicotine [J].
Beene, DL ;
Brandt, GS ;
Zhong, WG ;
Zacharias, NM ;
Lester, HA ;
Dougherty, DA .
BIOCHEMISTRY, 2002, 41 (32) :10262-10269
[3]   Probing protein electrostatics with a synthetic fluorescent amino acid [J].
Cohen, BE ;
McAnaney, TB ;
Park, ES ;
Jan, YN ;
Boxer, SG ;
Jan, LY .
SCIENCE, 2002, 296 (5573) :1700-1703
[4]   A NEW TOOL FOR STUDYING PROTEIN-STRUCTURE AND FUNCTION [J].
CORNISH, VW ;
SCHULTZ, PG .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1994, 4 (04) :601-607
[5]  
Dang H, 2000, MOL PHARMACOL, V57, P1114
[6]   Unnatural amino acids as probes of protein structure and function [J].
Dougherty, DA .
CURRENT OPINION IN CHEMICAL BIOLOGY, 2000, 4 (06) :645-652
[7]   Cation-pi interactions in chemistry and biology: A new view of benzene, Phe, Tyr, and Trp [J].
Dougherty, DA .
SCIENCE, 1996, 271 (5246) :163-168
[8]   Site-specific incorporation of (aminooxy)acetic acid into proteins [J].
Eisenhauer, BM ;
Hecht, SM .
BIOCHEMISTRY, 2002, 41 (38) :11472-11478
[9]   Mapping disulfide connectivity using backbone ester hydrolysis [J].
England, PM ;
Lester, HA ;
Dougherty, DA .
BIOCHEMISTRY, 1999, 38 (43) :14409-14415
[10]   Backbone mutations in transmembrane domains of a ligand-gated ion channel: Implications for the mechanism of gating [J].
England, PM ;
Zhang, YN ;
Dougherty, DA ;
Lester, HA .
CELL, 1999, 96 (01) :89-98
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