Unnatural amino acid mutagenesis in mapping ion channel function

被引：82

作者：

Beene, DL ^{[1
]}

Dougherty, DA

Lester, HA

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] CALTECH, Div Biol, Pasadena, CA 91125 USA

来源：

CURRENT OPINION IN NEUROBIOLOGY | 2003年 / 13卷 / 03期

关键词：

D O I：

10.1016/S0959-4388(03)00068-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure-function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing experiments on ion channels.

引用

页码：264 / 270

页数：7

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