Electrospun Carbon Nanotube-Based Scaffolds Exhibit High Conductivity and Cytocompatibility for Tissue Engineering Applications

被引:14
作者
Suh, Taylor C. [1 ]
Twiddy, Jack [2 ,3 ]
Mahmood, Nasif [1 ]
Ali, Kiran M. [1 ]
Lubna, Mostakima M. [1 ]
Bradford, Philip D. [1 ]
Daniele, Michael A. [2 ,3 ,4 ]
Gluck, Jessica M. [1 ]
机构
[1] North Carolina State Univ, Dept Text Engn Chem & Sci, Raleigh, NC 27606 USA
[2] North Carolina State Univ, Joint Dept Biomed Engn, Raleigh, NC 27606 USA
[3] Univ North Carolina Chapel Hill, Raleigh, NC 27606 USA
[4] North Carolina State Univ, Dept Elect & Comp Engn, Raleigh, NC 27606 USA
来源
ACS OMEGA | 2022年 / 7卷 / 23期
基金
美国国家科学基金会;
关键词
ELECTRICAL-STIMULATION; STEM-CELLS; DIFFERENTIATION; CARDIOMYOCYTES; MATURATION; NANOFIBERS; DIAMETER; BIOCOMPATIBILITY; PROLIFERATION; BIOMATERIALS;
D O I
10.1021/acsomega.2c01807
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Carbon nanotubes (CNTs) are known for their excellent conductive properties. Here, we present two novel methods, "sandwich" (sCNT) and dual deposition (DD CNT), for incorporating CNTs into electrospun polycaprolactone (PCL) and gelatin scaffolds to increase their conductance. Based on CNT percentage, the DD CNT scaffolds contain significantly higher quantities of CNTs than the sCNT scaffolds. The inclusion of CNTs increased the electrical conductance of scaffolds from 0.0 +/- 0.00 kS (non-CNT) to 0.54 +/- 0.10 kS (sCNT) and 5.22 +/- 0.49 kS (DD CNT) when measured parallel to CNT arrays and to 0.25 +/- 0.003 kS (sCNT) and 2.85 +/- 1.12 (DD CNT) when measured orthogonally to CNT arrays. The inclusion of CNTs increased fiber diameter and pore size, promoting cellular migration into the scaffolds. CNT inclusion also decreased the degradation rate and increased hydrophobicity of scaffolds. Additionally, CNT inclusion increased Young's modulus and failure load of scaffolds, increasing their mechanical robustness. Murine fibroblasts were maintained on the scaffolds for 30 days, demonstrating high cytocompatibility. The increased conductivity and high cytocompatibility of the CNT-incorporated scaffolds make them appropriate candidates for future use in cardiac and neural tissue engineering.
引用
收藏
页码:20006 / 20019
页数:14
相关论文
共 50 条
[31]   Collagen-carbon nanotube composite materials as scaffolds in tissue engineering [J].
MacDonald, RA ;
Laurenzi, BF ;
Viswanathan, G ;
Ajayan, PM ;
Stegemann, JP .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2005, 74A (03) :489-496
[32]   Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications [J].
Sell, Scott A. ;
Wolfe, Patricia S. ;
Ericksen, Jeffery J. ;
Simpson, David G. ;
Bowlin, Gary L. .
TISSUE ENGINEERING PART A, 2011, 17 (21-22) :2723-2737
[33]   Bioactive Electrospun Scaffolds Delivering Growth Factors and Genes for Tissue Engineering Applications [J].
Ji, Wei ;
Sun, Yan ;
Yang, Fang ;
van den Beucken, Jeroen J. J. P. ;
Fan, Mingwen ;
Chen, Zhi ;
Jansen, John A. .
PHARMACEUTICAL RESEARCH, 2011, 28 (06) :1259-1272
[34]   Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering [J].
Sang, Shengbo ;
Cheng, Rong ;
Cao, Yanyan ;
Yan, Yayun ;
Shen, Zhizhong ;
Zhao, Yajing ;
Han, Yanqing .
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B, 2022, 23 (01) :58-73
[35]   Engineering in-plane mechanics of electrospun polyurethane scaffolds for cardiovascular tissue applications [J].
Luketich, Samuel K. ;
Cosentino, Federica ;
Di Giuseppe, Marzio ;
Menallo, Giorgio ;
Nasello, Gabriele ;
Livreri, Patrizia ;
Wagner, William R. ;
D'Amore, Antonio .
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, 2022, 128
[36]   Nanocomposite Electrospun Scaffold Based on Polyurethane/Polycaprolactone Incorporating Gold Nanoparticles and Soybean Oil for Tissue Engineering Applications [J].
Asadi, Nahideh ;
Del Bakhshayesh, Azizeh Rahmani ;
Sadeghzadeh, Hadi ;
Asl, Amir Nezami ;
Kaamyabi, Sharif ;
Akbarzadeh, Abolfazl .
JOURNAL OF BIONIC ENGINEERING, 2023, 20 (04) :1712-1722
[37]   Physicochemical properties and cytocompatibility assessment of non-degradable scaffolds for bone tissue engineering applications [J].
Pereira, H. ;
Cengiz, I. F. ;
Maia, F. R. ;
Bartolomeu, F. ;
Oliveira, J. M. ;
Reis, R. L. ;
Silva, F. S. .
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, 2020, 112
[38]   Combination of polydopamine and carbon nanomaterials coating enhances the piezoelectric responses and cytocompatibility of biodegradable PLLA nanofiber scaffolds for tissue engineering applications [J].
Ramasamy, Madeshwaran Sekkarapatti ;
Bhaskar, Rakesh ;
Narayanan, Kannan Badri ;
Purohit, Shiv Dutt ;
Park, Sang Shin ;
Manikkavel, Amutheesan ;
Kim, Byungki ;
Han, Sung Soo .
MATERIALS TODAY COMMUNICATIONS, 2022, 33
[39]   Electrospun Scaffolds Based on Poly(butyl cyanoacrylate) for Tendon Tissue Engineering [J].
Bianchi, Eleonora ;
Vigani, Barbara ;
Ruggeri, Marco ;
Del Favero, Elena ;
Ricci, Caterina ;
Grisoli, Pietro ;
Ferraretto, Anita ;
Rossi, Silvia ;
Viseras, Cesar ;
Sandri, Giuseppina .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (04)
[40]   Effects of mechanical properties of carbon-based nanocomposites on scaffolds for tissue engineering applications: a comprehensive review [J].
Eivazzadeh-Keihan, Reza ;
Sadat, Zahra ;
Lalebeigi, Farnaz ;
Naderi, Nooshin ;
Panahi, Leila ;
Ganjali, Fatemeh ;
Mahdian, Sakineh ;
Saadatidizaji, Zahra ;
Mahdavi, Mohammad ;
Chidar, Elham ;
Soleimani, Erfan ;
Ghaee, Azadeh ;
Maleki, Ali ;
Zare, Iman .
NANOSCALE ADVANCES, 2024, 6 (02) :337-366