Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves

被引：41

作者：

Li, Zhengguo ^{[1
,2
]}

An, Lijun ^{[1
,2
]}

Zhang, Shaojie ^{[1
,2
]}

Shi, Zhaoyu ^{[1
,2
]}

Bao, Jiahe ^{[1
,2
]}

Tuerhong, Muhetaer ^{[3
]}

Abudukeremu, Munira ^{[3
]}

Xu, Jing ^{[1
,2
]}

Guo, Yuanqiang ^{[1
,2
]}

机构：

[1] Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300350, Peoples R China

[2] Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300350, Peoples R China

[3] Kashgar Univ, Lab Xinjiang Native Med & Edible Plant Resources, Coll Chem & Environm Sci, Kashgar 844000, Peoples R China

来源：

FOOD CHEMISTRY | 2021年 / 364卷

基金：

中国国家自然科学基金;

关键词：

Structural property; Immunomodulatory activity; Heteropolysaccharide; Stevia rebaudiana; Zebrafish model; PHYSICOCHEMICAL PROPERTIES; ANTIOXIDANT ACTIVITIES; IN-VITRO; INULIN; OLIGOSACCHARIDES; MACROPHAGES;

D O I：

10.1016/j.foodchem.2021.130310

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields. However, a few studies were performed to explore polysaccharides in this plant. Herein, SRP70-1 was derived from S. rebaudiana. Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da. SRP70-1 was found to contain -> 5)-alpha-L-Araf-(1 ->, -> 2,3,5)-alpha-L-Araf-(1 ->, -> 4)-beta-L-Arap-(1 ->, -> 4)-beta-D-Galp-(1 ->, -> 6)-beta-D-Galp-(1 ->, -> 4)-beta-D-Manp-(1 ->, -> 6)-beta-D-Manp-(1 ->, and terminal alpha-L-Araf, beta-D-Galp, and beta-D-Glcp residues. Cell experiments showed that SRP70-1 could significantly promote phagocytosis and increase the release of nitric oxide and cytokines including IL-1 beta, IL-6, and TNF-alpha. Further zebrafish experiments confirmed the immunological enhancement effects of SRP70-1. This study revealed that SRP70-1 may be useful for the development of functional foods.

引用

页数：9

共 52 条

[1] Effects of dietary mono- and multiprobiotic strains on growth performance, gut bacteria and body composition of Java']Javanese carp (Puntius gonionotus, Bleeker 1850) [J].

Allameh, S. K. ;

Yusoff, F. M. ;

Ringo, E. ;

Daud, H. M. ;

Saad, C. R. ;

Ideris, A. .

AQUACULTURE NUTRITION, 2016, 22 (02) :367-373

[2] Influence of the uronic acid composition on the gastroprotective activity of alginates from three different genus of Tunisian brown algae [J].

Ammar, Hiba Hadj ;

Lajili, Sirine ;

Sakly, Nawfel ;

Cherif, Dora ;

Rihouey, Christophe ;

Le Cerf, Didier ;

Bouraoui, Abderrahman ;

Majdoub, Hatem .

FOOD CHEMISTRY, 2018, 239 :165-171

[3] Structure and antiviral activity of arabinogalactan with (1→6)-β-D-galactan core from Stevia rebaudiana leaves [J].

Braz de Oliveira, Arildo Jose ;

Cordeiro, Lucimara M. C. ;

Correia Goncalves, Regina Aparecida ;

Ceole, Ligia Fernanda ;

Ueda-Nakamura, Tania ;

Iacomini, Marcello .

CARBOHYDRATE POLYMERS, 2013, 94 (01) :179-184

[4] Structural studies on hairy region of pectic polysaccharide from campion Silene vulgaris (Oberna behen) [J].

Bushneva, OA ;

Ovodova, RG ;

Shashkov, AS ;

Ovodov, YS .

CARBOHYDRATE POLYMERS, 2002, 49 (04) :471-478

[5] Structural characterization, in vitro and in vivo antioxidant activities of a heteropolysaccharide from the fruiting bodies of Morchella esculenta [J].

Cai, Zheng-Nan ;

Li, Wan ;

Mehmood, Shomaila ;

Pan, Wen-Juan ;

Wang, Ya ;

Meng, Fan-Ju ;

Wang, Xiao-Fang ;

Lu, Yong-Ming ;

Chen, Yan .

CARBOHYDRATE POLYMERS, 2018, 195 :29-38

[6] Isolation and prebiotic activity of inulin-type fructan extracted from Pfaffia glomerata (Spreng) Pedersen roots [J].

Caleffi, Edilainy Rizzieri ;

Krausova, Gabriela ;

Hyrslova, Ivana ;

Ramos Paredes, Larry Ladislao ;

dos Santos, Marcelo Mueller ;

Sassaki, Guilherme Lanzi ;

Correia Goncalves, Regina Aparecida ;

Braz de Oliveira, Arildo Jose .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 80 :392-399

[7] Structural Elucidation of a Novel Pectin-Polysaccharide from the Petal of Saussurea laniceps and the Mechanism of its Anti-HBV Activity [J].

Chen, Wenbo ;

Zhu, Xiaolu ;

Ma, Juanjuan ;

Zhang, Mengmeng ;

Wu, Hui .

CARBOHYDRATE POLYMERS, 2019, 223

[8] Solution NMR Spectroscopy of Food Polysaccharides [J].

Cheng, H. N. ;

Neiss, Thomas G. .

POLYMER REVIEWS, 2012, 52 (02) :81-114

[9] A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].

CIUCANU, I ;

KEREK, F .

CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217

[10] NMR structural elucidation of the arabinan from Prunus dulcis immunobiological active pectic polysaccharides [J].

Dourado, Fernando ;

Cardoso, Susana M. ;

Silva, Artur M. S. ;

Gama, Francisco M. ;

Coimbra, Manuel A. .

CARBOHYDRATE POLYMERS, 2006, 66 (01) :27-33

← 1 2 3 4 5 6 →