Effect of desensitizing agents on the microtensile bond strength of two-step etch-and-rinse adhesives to dentin

Desensitizers can be used to control postoperative sensitivity in adhesive restorations. The aim of this study was to evaluate the effect of desensitizing agents on the bond strength of two-step etch-and-rinse adhesive systems to dentin. Forty-two human molars were sectioned to obtain 3-mm-thick dentin discs. The discs were divided into three groups (n = 14 in each) -no-treatment control group (CT), and oxalic acid [BisBlock (BB)] and calcium phosphate [Desensibilize Nano-P (NP)] desensitizers -before the application of two adhesive systems [Adper Single Bond Plus (SB) and One-Step Plus (OSP)]. A nanoparticle composite resin was used to create a 3-mm-thick build-up. The specimens were stored in distilled water for 24 h before a microtensile bond-strength test was performed. The failure modes were determined using a stereomicroscope at 100 x magnification. Specimens were sectioned perpendicular to the interface for scanning electron microscopy analyses. The CT-SB group exhibited the highest bond strength, differing significantly from BBSB and BB-OSP groups. Mixed failures were prevalent for all groups. Scanning electron microscopy revealed a continuous hybrid layer and resin tags in all groups. Dentin bond strength of etch-and-rinse adhesive systems was reduced by an oxalic acid desensitizer but was not affected by a calcium phosphate-containing desensitizer.