The protection of CpG ODNs and Yarrowia lipolytica harboring VP28 for shrimp Litopenaeus vannamei against White spot syndrome virus infection

被引:0
作者
Yi, Q. [1 ,2 ]
Liu, R. [1 ]
Sun, R. [1 ,2 ]
Wang, L. [1 ]
Zhou, Z. [1 ]
Wang, M. [1 ]
Liu, Y. [3 ]
Sun, J. [3 ]
Madzak, C. [4 ]
Song, L. [1 ]
机构
[1] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China
[4] INRA, CNRS, INAPG, Microbiol & Genet Mol UMR1238,CBAI, F-78850 Thiverval Grignon, France
来源
ISJ-INVERTEBRATE SURVIVAL JOURNAL | 2014年 / 11卷
关键词
CpG ODNs; Yarrowia lipolytica surface-display VP28; White spot syndrome virus; Litopenaeus vannamei; disease resistance; antiviral immunity; SCALLOP CHLAMYS-FARRERI; ENVELOPE PROTEIN VP28; IMMUNE-RESPONSES; INNATE IMMUNITY; NITRIC-OXIDE; ANTIVIRAL IMMUNITY; PENAEUS-MONODON; BACTERIAL-DNA; EXPRESSION; STAT;
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The white spot syndrome is one of the most serious disease which has caused high mortalities and huge economic losses to shrimp culture. In the present study, the oral administrations with CpG ODNs and Yarrowia lipolytica harboring VP28 (rVP28-yl) as dietary supplement for shrimp Litopenaeus vannamei were conducted to evaluate their protective effects against WSSV. After feeding for 15 days, the cumulative mortality and the copy number of WSSV in CpG and rVP28-yl feeding shrimps were significantly lower when they were challenged by WSSV, compared with those in control shrimps (p < 0.05). The caspase-3 activity was suppressed in rVP28-yl feeding shrimps but ascended in CpG feeding shrimps after WSSV challenge. Besides, the PO activity in CpG feeding shrimps was significantly increased after feeding trial, and kept increasing post WSSV challenge (p < 0.05). While the increased NO production was observed both in CpG and rVP28-yl feeding shrimps after feeding trial and WSSV challenge. In addition, increased mRNA expression levels of STAT and Dicer were observed in CpG group post WSSV challenge. These results together indicated that oral feeding of CpG ODNs and rVP28-yl could enhance the innate non-specific immune responses especially antiviral immunity of shrimps in varying degrees, and increase their resistance against WSSV infection.
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页码:119 / 131
页数:13
相关论文
共 57 条
[51]   CLONING OF THE LYS5-GENE ENCODING SACCHAROPINE DEHYDROGENASE FROM THE YEAST YARROWIA-LIPOLYTICA BY TARGET INTEGRATION [J].
XUAN, JW ;
FOURNIER, P ;
GAILLARDIN, C .
CURRENT GENETICS, 1988, 14 (01) :15-21
[52]  
Yi GH, 2004, J BIOCHEM MOL BIOL, V37, P726
[53]   Screening the organs for early detection of white spot syndrome virus in Penaeus indicus by histopathology and PCR techniques [J].
Yoganandhan, K ;
Sathish, S ;
Murugan, V ;
Narayanan, RB ;
Hameed, ASS .
AQUACULTURE, 2003, 215 (1-4) :21-29
[54]   Construction of a new plasmid for surface display on cells of Yarrowia lipolytica [J].
Yue, Lixi ;
Chi, Zhenming ;
Wang, Lin ;
Liu, Jia ;
Madzak, Catherine ;
Li, Jing ;
Wang, Xianghong .
JOURNAL OF MICROBIOLOGICAL METHODS, 2008, 72 (02) :116-123
[55]   Protective immunity induced by CpG ODNs against white spot syndrome virus (WSSV) via intermediation of virus replication indirectly in Litopenaeus vannamei [J].
Zhang, Ying ;
Song, Linsheng ;
Zhao, Jianmin ;
Wang, Lingling ;
Kong, Pengfei ;
Liu, Lin ;
Wang, Mengqiang ;
Qiu, Limei .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2010, 34 (04) :418-424
[56]   The increased transcriptional response and translocation of a Rel/NF-κB homologue in scallop Chlamys farreri during the immune stimulation [J].
Zhou, Zhi ;
Wang, Mengqiang ;
Zhao, Jianmin ;
Wang, Lingling ;
Gao, Yang ;
Zhang, Huan ;
Liu, Rui ;
Song, Linsheng .
FISH & SHELLFISH IMMUNOLOGY, 2013, 34 (05) :1209-1215
[57]   A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri [J].
Zhou, Zhi ;
Yang, Jialong ;
Wang, Lingling ;
Zhang, Huan ;
Gao, Yang ;
Shi, Xiaowei ;
Wang, Mengqiang ;
Kong, Pengfei ;
Qiu, Limei ;
Song, Linsheng .
PLOS ONE, 2011, 6 (04)