Two point resolution in incoherent imaging

被引:8
作者
Heintzmann, R [1 ]
Sarafis, V
机构
[1] Max Planck Inst Biophys Chem, Dept Biol Mol, D-37077 Gottingen, Germany
[2] Univ Queensland, Sch Comp & Elect Engn, St Lucia, Qld 4072, Australia
来源
OPTIK | 2001年 / 112卷 / 03期
关键词
sparrow criterion; two point resolution; Point Spread Function; vector theory; incoherent imaging; projection;
D O I
10.1078/0030-4026-00022
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
The formalism for incoherent imaging of two points separated by the Sparrow criterion is investigated in two and three dimensions. Two and three dimensional imaging enables us to distinguish between a single point object and a two point object better than from the one dimensional information. Projections of the two point object, performed by integrating along the axial direction, are shown to improve the observed resolution especially for low NA non confocal systems. There are however a number of different objects, whose images are shown to be virtually indistinguishable. Therefore, resolution measures should only be used in the presence of knowledge about the class of objects being imaged.
引用
收藏
页码:114 / 118
页数:5
相关论文
共 10 条
[1]  
Born M., 1986, PRINCIPLES OPTICS
[2]   Equivalence of the Huygens-Fresnel and Debye approach for the calculation of high aperture point-spread functions in the presence of refractive index mismatch [J].
Egner, A ;
Hell, SW .
JOURNAL OF MICROSCOPY-OXFORD, 1999, 193 :244-249
[3]  
FRIGO M, 1999, FASTEST FOURIER TRAN
[4]   RESOLUTION IN LIGHT-MICROSCOPY STUDIED BY COMPUTER-SIMULATION [J].
GOLDSTEIN, DJ .
JOURNAL OF MICROSCOPY-OXFORD, 1992, 166 :185-197
[5]   Reconstruction of axial tomographic high resolution data from confocal fluorescence microscopy: a method for improving 3D FISH images [J].
Heintzmann, R ;
Kreth, G ;
Cremer, C .
ANALYTICAL CELLULAR PATHOLOGY, 2000, 20 (01) :7-15
[6]  
LIPSON T, 1997, OPTIK
[7]   ELECTROMAGNETIC DIFFRACTION IN OPTICAL SYSTEMS .2. STRUCTURE OF THE IMAGE FIELD IN AN APLANATIC SYSTEM [J].
RICHARDS, B ;
WOLF, E .
PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1959, 253 (1274) :358-379
[8]   Contrast, resolution, pixelation, dynamic range and signal-to-noise ratio: fundamental limits to resolution in fluorescence light microscopy [J].
Stelzer, EHK .
JOURNAL OF MICROSCOPY-OXFORD, 1998, 189 :15-24
[9]   The role of specimen-induced spherical aberration in confocal microscopy [J].
Török, P ;
Hewlett, SJ ;
Varga, P .
JOURNAL OF MICROSCOPY-OXFORD, 1997, 188 :158-172
[10]   ELECTROMAGNETIC DIFFRACTION IN OPTICAL SYSTEMS .1. AN INTEGRAL REPRESENTATION OF THE IMAGE FIELD [J].
WOLF, E .
PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1959, 253 (1274) :349-357