Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene

被引：43

作者：

Song, Cuiping ^{[2
,3
]}

Zhu, Chao ^{[1
]}

Zhang, Chaofan ^{[1
]}

Cui, Shangjin ^{[1
]}

机构：

[1] CAAS, Harbin Vet Res Inst, Div Swine Infect Dis, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

[2] Ocean Univ China, Lab Marine Genet & Breeding MGB, Qingdao 266003, Peoples R China

[3] China Anim Hlth & Epidemiol Ctr, Qingdao 266032, Peoples R China

来源：

VIROLOGY JOURNAL | 2010年 / 7卷

关键词：

POLYMERASE-CHAIN-REACTION;

D O I：

10.1186/1743-422X-7-353

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the non-structural protein NS1 gene were designed. The detection limit was 1 x 10(2) DNA copies/mu L, and the assay was linear in the range of 1 x 10(2) to 1 x 10(9) copies/mu L. There was no cross-reaction with porcine circovirus 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), classical swine fever virus (CSFV), or Japanese encephalitis virus (JEV). The assay was specific and reproducible. In 41 clinical samples, PPV was detected in 32 samples with the real-time PCR assay and in only 11 samples with a conventional PCR assay. The real-time assay using the TaqMan-system can therefore be practically used for studying the epidemiology and management of PPV.

引用

页数：4

共 10 条

[1] STRUCTURE AND REPLICATION OF MINUTE VIRUS OF MICE DNA [J].