共 50 条
Dietary β-conglycinin prevents acute ethanol-induced fatty liver in mice
被引:4
|作者:
Ikaga, Reina
[1
]
Li, Dongyang
[1
,2
]
Yamazaki, Tomomi
[1
]
机构:
[1] Natl Inst Biomed Innovat Hlth & Nutr, Dept Nutr Sci, Natl Inst Hlth & Nutr, Shinjuku Ku, 1-23-1 Toyama, Tokyo 1628636, Japan
[2] Ochanomizu Univ, Grad Sch Human & Sci, Bunkyo Ku, 2-1-1 Otsuka, Tokyo 1128610, Japan
关键词:
beta-Conglycinin;
Ethanol-induced fatty liver;
Ethanol administration;
PPAR gamma;
SCD1;
ELEMENT-BINDING PROTEINS;
INDUCED HEPATIC STEATOSIS;
GENE-EXPRESSION;
PROTECTS MICE;
PPAR-GAMMA;
CHOLESTEROL;
ALCOHOL;
DISEASE;
INHIBITION;
METABOLISM;
D O I:
10.1016/j.bbrc.2017.08.155
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Alcoholic fatty liver is the earliest stage of alcohol-induced liver disease leading to liver cirrhosis. beta-Conglycinin, one of the soy proteins, is known to prevent non-alcoholic fatty liver, hyperlipidemia and obesity. Therefore, we examined whether beta-conglycinin feeding has an effect on the prevention of acute ethanol-induced fatty liver in mice. Male C57BL/6J mice were fed with 20 energy% beta-conglycinin or casein for 4 weeks prior to ethanol administration and were then given ethanol or glucose, as a control, by gavage. Ethanol significantly increased liver triglyceride (TG) in mice fed casein due to the activation of peroxisome proliferator-activated receptor (PPAR) gamma 2, a nuclear transcription factor known for regulating lipid metabolism and de novo lipogenesis. The liver TG of ethanol-administered beta-conglycinin-fed mice was significantly lower than that in those fed casein, although ethanol increased the amount of liver TG in mice fed beta-conglycinin. The increased levels of PPAR gamma 2 protein and its target gene CD36 in response to an ethanol were not observed in mice fed beta-conglycinin. Moreover, beta-conglycinin decreased the basal expression of de novo lipogenesis-related genes such as stearoyl-CoA desaturase-1, and therefore, the expressions of these genes were lower in the ethanol-administered beta-conglycinin-fed mice than in the casein-fed mice. In conclusion, beta-conglycinin supplementation appears to prevent the development of fatty liver in mice caused by ethanol consumption via the suppression of alcohol induced activation of PPAR gamma 2 and the downregulation of the basal expression of de novo lipogenesis. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码:542 / 547
页数:6
相关论文