Stimulating GABAergic Neurons in the Nucleus Accumbens Core Alters the Trigeminal Neuropathic Pain Responses in a Rat Model of Infraorbital Nerve Injury

被引：14

作者：

Islam, Jaisan ^{[1
]}

Elina, K. C. ^{[1
]}

Kim, Soochong ^{[2
]}

Kim, Hyong Kyu ^{[3
]}

Park, Young Seok ^{[1
,2
,4
]}

机构：

[1] Chungbuk Natl Univ, Coll Med, Dept Med Neurosci, Cheongju 28644, South Korea

[2] Chungbuk Natl Univ, Coll Vet Med, Dept Vet Med, ISCRM, Cheongju 28644, South Korea

[3] Chungbuk Natl Univ, Coll Med, Dept Med & Microbiol, Cheongju 28644, South Korea

[4] Chungbuk Natl Univ Hosp, Dept Neurosurg, Cheongju 28644, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 16期

基金：

新加坡国家研究基金会;

关键词：

microdialysis; nucleus accumbens core; optogenetics; trigeminal neuralgia; VPM thalamus; CHRONIC CONSTRICTION INJURY; EPIGENETIC MECHANISMS; CINGULATE CORTEX; AMPA RECEPTORS; DOPAMINE; NOCICEPTION; CIRCUITRY; REWARD; INVOLVEMENT; MORPHINE;

D O I：

10.3390/ijms22168421

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleus accumbens core (NAcc) is an important component of brain reward circuitry, but studies have revealed its involvement in pain circuitry also. However, its effect on trigeminal neuralgia (TN) and the mechanism underlying it are yet to be fully understood. Therefore, this study aimed to examine the outcomes of optogenetic stimulation of NAcc GABAergic neurons in an animal model of TN. Animals were allocated into TN, sham, and control groups. TN was generated by infraorbital nerve constriction and the optogenetic virus was injected into the NAcc. In vivo extracellular recordings were acquired from the ventral posteromedial nucleus of the thalamus. Alterations of behavioral responses during stimulation "ON" and "OFF" conditions were evaluated. In vivo microdialysis was performed in the NAcc of TN and sham animals. During optogenetic stimulation, electrophysiological recordings revealed a reduction of both tonic and burst firing activity in TN animals, and significantly improved behavioral responses were observed as well. Microdialysis coupled with liquid chromatography/tandem mass spectrometry analysis revealed significant alterations in extracellular concentration levels of GABA, glutamate, acetylcholine, dopamine, and citrulline in NAcc upon optic stimulation. In fine, our results suggested that NAcc stimulation could modulate the transmission of trigeminal pain signals in the TN animal model.

引用

页数：18

共 70 条

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