Development of natural cellulase inhibitor mediated intensified biological pretreatment technology using Pleurotus florida for maximum recovery of cellulose from paddy straw under solid state condition

被引:22
作者
Kumar, Manickam Naresh [1 ]
Ravikumar, Rajarathinam [1 ]
Thenmozhi, Senniyappan [1 ]
Sankar, Muthuvelu Kirupa [1 ]
机构
[1] Bannari Amman Inst Technol, Dept Biotechnol, Bioenergy Res Lab, Erode 638401, TN, India
关键词
Paddy straw; Natural cellulase inhibitor; Cellulose recovery; Laccase; ATR-FTIR; WHITE-ROT FUNGI; ENZYMATIC-HYDROLYSIS; WHEAT-STRAW; RICE STRAW; LIGNOCELLULOSIC BIOMASS; BIOETHANOL; SACCHARIFICATION; FERMENTATION; OPTIMIZATION; SUBSTRATE;
D O I
10.1016/j.biortech.2017.07.105
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignification by 1.2 and 1.5-fold respectively, compared to conventional bio-pretreatment due to inhibition of 61% of cellulase activity in IMBP. Further increase in NCI concentration showed negative effect on Pleurotus florida growth and suppress the laccase productivity by 1.1-fold. Laccase activity in IMBP was found to be 2.0 U/mL on 19th day, which is higher than (1.5 U/mL) conventional bio-pretreatment. Physico-chemical modifications in paddy straw before and after pretreatment were analysed by SEM, ATR-FTIR, XRD and TGA. According to these findings, the IMBP technology can be a viable eco-friendly technology for sustainable production of bioethanol with maximum cellulose recovery.
引用
收藏
页码:353 / 361
页数:9
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