The ESR method to determine nitric oxide in plants

被引：6

作者：

Xu, YC ^{[1
]}

Cao, YL ^{[1
]}

Tao, Y ^{[1
]}

Zhao, BL ^{[1
]}

机构：

[1] Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China

来源：

NITRIC OXIDE, PT E | 2005年 / 396卷

关键词：

D O I：

10.1016/S0076-6879(05)96009-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitric oxide (NO) plays an important role not only in animal system but also in plant system. We developed a method to detect NO free radical in plant system using electron spin resonance (ERS) spin trapping technique. The adduct (DETC)(2)-Fe(2+)-NO in plant can be extracted by organic solvent and then measured on an ESR spectrometer at room temperature (indirect method) or can be measured in live plant on an ESR spectrometer, but the water in the plant has to be partially dehydrated (direct method).

引用

页码：84 / 92

页数：9

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