Design, synthesis and evaluation of a molecularly imprinted polymer for hollow fiber-solid phase microextraction of chlorogenic acid in medicinal plants

被引:59
作者
Golsefidi, Mazyar Ahmadi [1 ,2 ]
Es'haghi, Zarrin [2 ]
Sarafraz-Yazdi, Ali [3 ]
机构
[1] Islamic Azad Univ, Dept Chem, Fac Sci, Gorgan Branch, Gorgan, Iran
[2] Payame Noor Univ, Dept Chem, Tehran 193954697, Iran
[3] Ferdowsi Univ Mashhad, Dept Chem, Fac Sci, Mashhad, Iran
关键词
Chlorogenic acid; Hollow fiber solid phase microextraction; Molecularly imprinted polymers; PERFORMANCE LIQUID-CHROMATOGRAPHY; GEL; PERVAPORATION; EXTRACTION; MEMBRANES;
D O I
10.1016/j.chroma.2012.01.019
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this study, a simple preparation approach was developed for modified bisphenol A (BPA) molecularly imprinted polymer sorbent used in the hollow fiber solid phase microextraction (MIP-HF-SPME) of chlorogenic acid (CGA). The pre-polymer solution containing the template was introduced into the polypropylene hollow fiber segment for in situ polymerization. MIP-HF-SPME conditions based on the modified MIP-sorbent were optimized. Finally, the tool was used for selective extraction of chlorogenic acid in Echinacea purpurea, a medicinal plant. Main parameters affecting synthesis of organic-inorganic hybrid MIP and microextraction procedure were investigated and optimized. The measurements were done under the optimal conditions. The limit of detection has been gained 0.08 ng/mL. The linear range and relative standard deviation (RSD %) are 0.2-1000 ng/mL and 0.38 (n = 3) respectively. The average relative recoveries of spiked analyte in the four concentration levels were between 84.8 and 97.2%. (C) 2012 Elsevier B.V. All rights reserved.
引用
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页码:24 / 29
页数:6
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