Changes and Function of Circulating Endothelial Progenitor Cells in Patients With Cerebral Aneurysm

被引：42

作者：

Wei, Huijie ^{[1
,2
]}

Mao, Qun ^{[1
,2
]}

Liu, Li ^{[2
]}

Xu, Yong ^{[1
,2
]}

Chen, Jieli ^{[3
]}

Jiang, Rongcai ^{[1
,2
]}

Yin, Long ^{[4
]}

Fan, Yimu ^{[4
]}

Chopp, Michael ^{[3
,5
]}

Dong, Jingfei ^{[6
]}

Zhang, Jianning ^{[1
,2
]}

机构：

[1] Tianjin Med Univ Gen Hosp, Dept Neurosurg, Tianjin, Peoples R China

[2] Tianjin Key Lab Injuries Variat & Regenerat Nervo, Tianjin Neurol Inst, Key Lab Posttrauma Neurorepair & Regenerat Cent N, Minist Educ, Tianjin, Peoples R China

[3] Henry Ford Hlth Syst, Dept Neurol, Detroit, MI USA

[4] Tianjin HuanHu Hosp, Dept Neurosurg, Tianjin, Peoples R China

[5] Oakland Univ, Dept Phys, Rochester, MI USA

[6] Baylor Coll Med, Dept Med, Thrombosis Res Sect, Houston, TX 77030 USA

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 2011年 / 89卷 / 11期

基金：

中国国家自然科学基金;

关键词：

endothelial progenitor cells; cerebral aneurysm; endothelial cells; vascular endothelial growth factor (VEGF); DECREASED NUMBER; SHEAR-STRESS; GROWTH; THERAPY; REENDOTHELIALIZATION; PROLIFERATION; MOBILIZATION; DYSFUNCTION; EXPRESSION; INCREASE;

D O I：

10.1002/jnr.22696

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Endothelial dysfunction is a trigger for the formation of cerebral aneurysm (CA). The circulating endothelial progenitor cell (EPC) plays an important role in postnatal vasculogenesis and reduction of endothelial injury. In this study, we tested the hypothesis that decreased number and impaired function of circulating EPCs correlate with CA formation in patients. Blood circulating EPCs were identified by flow cytometry. The level of plasma vascular endothelial growth factor (VEGF) was measured by ELISA. Circulating EPCs from patients (n = 27) were cultured in vitro, and the function of EPCs was evaluated by cell migration and senescence-associated p-galactosidase activity. The number of circulating EPCs was significantly decreased in both unruptured and ruptured CA patients compared with healthy control subjects. Impaired migratory capacity and elevated cellular senescence of cultured EPCs were observed in patients with CA (ruptured and unruptured). The percentages of EPC senescence in patients with CAs were significantly and negatively correlated with the number of circulating EPCs. In addition, there were higher levels of plasma VEGF in CA patients compared with healthy control subjects. Our results show that the numbers and functions of circulating EPCs are reduced in patients with CAs. These findings suggest that the decreased number and impaired function of circulating EPCs in CA patients may contribute to the pathophysiological process of aneurysm formation. (C) 2011 Wiley-Liss, Inc.

引用

页码：1822 / 1828

页数：7

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