Antigenic cross-reactivity among human, swine, rabbit and avian hepatitis E virus capsid proteins

被引：0

作者：

Sun, Yani ^{[1
,2
]}

Yan, Wenlong ^{[1
,2
]}

Chen, Xu ^{[1
,2
]}

Liu, Qianqian ^{[1
,2
]}

Ji, Pinpin ^{[1
,2
]}

Zhu, Jiahong ^{[1
,2
]}

Gai, Lili ^{[3
]}

Li, Xiaoxuan ^{[1
,2
]}

Zhao, Jiakai ^{[1
,2
]}

Zhang, Lu ^{[1
,2
]}

Zhang, Hao ^{[1
,2
]}

Liu, Baoyuan ^{[1
,2
]}

Zhou, En-Min ^{[1
,2
]}

Zhao, Qin ^{[1
,2
]}

机构：

[1] Northwest A&F Univ, Dept Vet Prevent Med, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China

[2] Minist Agr, Sci Observing & Expt Stn Vet Pharmacol & Diagnost, Yangling 712100, Shaanxi, Peoples R China

[3] Shandong Bluetown Anal & Test Co Ltd, Jinan 250102, Shandong, Peoples R China

来源：

VETERINARY MICROBIOLOGY | 2022年 / 265卷

基金：

中国国家自然科学基金;

关键词：

Hepatitis E virus; Capsid protein; Cross-reactivity; Antigenic relation; Common epitopes; HEV; GENOTYPE; INFECTION; CHICKENS; EPITOPES; ELISA;

D O I：

10.1016/j.vetmic.2022.109331

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis E virus (HEV), a zoonotic virus, infects many animal species, including humans. Capsid proteins of human, swine, rabbit and avian HEVs share 48 %-100 % amino acid identity. In the present study, antigenic cross-reactivity among human, swine, rabbit and avian HEV capsid proteins were analyzed in detail using indirect and blocking enzyme-linked immunosorbent assays (ELISAs). The C-terminal 268 amino acids of genotype 1 human, genotype 4 swine, genotype 3 rabbit and genotype B3 avian HEV capsid proteins served as coating antigens for ELISA. Hyperimmune rabbit antisera (against four HEV capsid proteins) and human, pig, rabbit and chicken clinical sera were as primary antibodies. Closely correlated and statistically indistinguishable results were obtained for detection of anti-HEV antibodies in human and pig sera using human, swine and rabbit HEV capsid proteins as coating antigens. Moderately correlated differences in detection of anti-HEV antibodies in rabbit sera were obtained using the three capsid proteins. Statistically significant differences with no correlations were obtained for anti-HEV antibodies detection in chicken sera between avian HEV capsid protein and human, swine and rabbit ones. Blocking ELISA results demonstrated that two common epitopes among the four species HEVs were immunodominant in avian HEV, but were non-immunodominant in human, swine and rabbit HEVs. Nevertheless, three epitopes common to human, swine and rabbit HEVs were all immunodominant epitopes for the three species HEVs. Collectively, these results demonstrate that anti-HEV antibodies in human and pig sera can be detected using human, swine and rabbit HEV capsid proteins. By contrast, for optimal detection of antiHEV antibodies in rabbit and chicken sera, the respective rabbit and avian HEV capsid proteins should be used. These results provide insights to guide future development of serological assays for diagnosing HEV infections in various animal species.

引用

页数：8

共 36 条

[1] Comparative sensitivity of commercial tests for hepatitis E genotype 3 virus antibody detection [J].

Avellon, Ana ;

Morago, Lucia ;

del Carmen Garcia-Galera, Maria ;

Munoz, Milagros ;

Echevarria, Jose-Manuel .

JOURNAL OF MEDICAL VIROLOGY, 2015, 87 (11) :1934-1939

[2] Sequence analysis and comparison of avian hepatitis E viruses from Australia and Europe indicate the existence of different genotypes [J].

Bilic, Lvana ;

Jaskulska, Barbara ;

Basic, Ana ;

Morrow, Chris J. ;

Hess, Michael .

JOURNAL OF GENERAL VIROLOGY, 2009, 90 :863-873

[3]

Chen YY, 2018, J VIROL, V92, DOI [10.1128/JVI.00251-18, 10.1128/jvi.00251-18]

[4] A Novel Blocking ELISA for Detection of Antibodies against Hepatitis E Virus in Domestic Pigs [J].

Chen, Yiyang ;

Zhao, Qin ;

Liu, Baoyuan ;

Wang, Lizhen ;

Sun, Yani ;

Li, Huixia ;

Wang, Xinjie ;

Syed, Shahid Faraz ;

Zhang, Gaiping ;

Zhou, En-Min .

PLOS ONE, 2016, 11 (03)

[5] A mutation in the progesterone receptor predisposes to HEV infection in HIV-positive patients [J].

Debes, Jose D. ;

Pas, Suzan D. ;

Groothuismink, Zwier M. A. ;

van der Ende, Marchina E. ;

de Man, Robert A. ;

Boonstra, Andre .

LIVER INTERNATIONAL, 2018, 38 (05) :792-796

[6] Hepatitis E virus infection in the HIV-positive patient [J].

Debes, Jose D. ;

Pisano, Maria Belen ;

Lotto, Martin ;

Re, Viviana .

JOURNAL OF CLINICAL VIROLOGY, 2016, 80 :102-106

[7] Zoonotic Hepatitis E Virus: Classification, Animal Reservoirs and Transmission Routes [J].

Doceul, Virginie ;

Bagdassarian, Eugenie ;

Demange, Antonin ;

Pavio, Nicole .

VIRUSES-BASEL, 2016, 8 (10)

[8] Analysis of epitopes in the capsid protein of avian hepatitis E virus by using monoclonal antibodies [J].

Dong, Shiwei ;

Zhao, Qin ;

Lu, Mingzhe ;

Sun, Peiming ;

Qiu, Hongkai ;

Zhang, Lu ;

Lv, Junhua ;

Zhou, En-Min .

JOURNAL OF VIROLOGICAL METHODS, 2011, 171 (02) :374-380

[9]

European Centre For Disease Prevention And Control,, 2017, FACTS HEP E

[10] Cross-Species Transmission of Swine Hepatitis E Virus Genotype 3 to Rabbits [J].

Han, Sang-Hoon ;

Park, Byung-Joo ;

Ahn, Hee-Seop ;

Kim, Yong-Hyun ;

Go, Hyeon-Jeong ;

Lee, Joong-Bok ;

Park, Seung-Yong ;

Song, Chang-Seon ;

Lee, Sang-Won ;

Choi, Yang-Kyu ;

Choi, In-Soo .

VIRUSES-BASEL, 2020, 12 (01)

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