Fluorescence visualization of cellulose and pectin in the primary plant cell wall

被引：50
作者：
Bidhendi, A. J. [1 ]
Chebli, Y. [1 ]
Geitmann, A. [1 ]
机构：
[1] McGill Univ, Dept Plant Sci, Macdonald Campus,21111 Lakeshore, Ste Anne De Bellevue, PQ H9X 3V9, Canada
来源：
JOURNAL OF MICROSCOPY | 2020年 / 278卷 / 03期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Cell development; cellulose; confocal laser scanning microscopy; fluorescence microscopy; immunohistochemistry; label; live-cell imaging; primary plant cell wall; pectin; polysaccharides; stain; CARBOHYDRATE-BINDING MODULES; POLLEN-TUBE; MONOCLONAL-ANTIBODIES; MICROFIBRIL ORIENTATION; CRYSTALLINE CELLULOSE; CALCOFLUOR WHITE; GUARD-CELLS; LIVE-CELL; GROWTH; POLYSACCHARIDES;
D O I：
10.1111/jmi.12895
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Plant cell walls constitute the extracellular matrix surrounding plant cells and are composed mainly of polysaccharides. The chemical makeup of the primary plant cell wall, and specifically, the abundance, localization and arrangement of the constituting polysaccharides are intimately linked with growth, morphogenesis and differentiation in plant cells. Visualization of the cell wall components is, therefore, a crucial tool in plant cell developmental studies. In this technical update, we present protocols for fluorescence visualization of cellulose and pectin in selected plant tissues and illustrate examples of some of the available labels that hold promise for live imaging of plant cell wall expansion and morphogenesis.
引用
收藏
页码：164 / 181
页数：18
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