CRISPR Technology in Cancer Diagnosis and Treatment: Opportunities and Challenges

被引:9
作者
Shademan, Behrouz [1 ]
Masjedi, Sepideh [2 ]
Karamad, Vahidreza [1 ]
Isazadeh, Alireza [3 ]
Sogutlu, Fatma [1 ]
Rad, Mohammad Hosein Saeedi [4 ]
Nourazarian, Alireza [5 ]
机构
[1] EGE Univ, Fac Med, Dept Med Biol, Izmir, Turkey
[2] Islamic Azad Univ, Dept Cellular & Mol Biol Sci, Tonekabon Branch, Tonekabon, Iran
[3] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran
[4] Shahid Beheshti Univ Med Sci, Dept Biol, Tehran, Iran
[5] Khoy Univ Med Sci, Dept Basic Med Sci, Khoy, Iran
关键词
CRISPR; Gene editing; Cancer treatment; Solid tumors; IN-VIVO; COLORECTAL-CANCER; LUNG-CANCER; CHROMOSOMAL TRANSLOCATIONS; TUMOR-SUPPRESSOR; DNA METHYLATION; GENE-EXPRESSION; HUMAN-CELLS; GENOME; CAS;
D O I
10.1007/s10528-022-10193-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel gene editing tool, the Cas system, associated with the CRISPR system, is emerging as a potential method for genome modification. This simple method, based on the adaptive immune defense system of prokaryotes, has been developed and used in human cancer research. These technologies have tremendous therapeutic potential, especially in gene therapy, where a patient-specific mutation is genetically corrected to cure diseases that cannot be cured with conventional treatments. However, translating CRISPR/Cas9 into the clinic will be challenging, as we still need to improve the efficiency, specificity, and application of the technology. In this review, we will explain how CRISPR-Cas9 technology can treat cancer at the molecular level, focusing on ordination and the epigenome. We will also focus on the promise and shortcomings of this system to ensure its application in the treatment and prevention of cancer.
引用
收藏
页码:1446 / 1470
页数:25
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