Analysis of human urine metabolites using SPE and NMR spectroscopy

被引：26

作者：

Yang WeiJin ^{[1
]}

Wang YaWei ^{[1
]}

Zhou QunFang ^{[1
]}

Tang HuiRu ^{[2
]}

机构：

[1] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China

[2] Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China

来源：

SCIENCE IN CHINA SERIES B-CHEMISTRY | 2008年 / 51卷 / 03期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

urine metabolites; NMR spectroscopy; solid phase extraction; metabonomics; metabolomics;

D O I：

10.1007/s11426-008-0031-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nuclear magnetic resonance (NMR) spectroscopic analysis of metabonome/metabolome has wide-spread applications in biomedical science researches. However, most of NMR resonances for urinary metabolites remain to be fully assigned. In the present study, human urine samples from two healthy volunteers were pre-treated with C18 solid-phase extraction and the resultant 5 sub-fractions were subjected to one-and two-dimensional NMR studies, including H-1 J-Resolved, H-1-H-1 COSY, H-1-H-1 TOCSY, H-1-C-13 HSQC, and HMBC 2D NMR. More than 70 low molecular weight metabolites were identified, and complete assignments of H-1 and C-13 resonances including many complex coupled spin systems were obtained.

引用

页码：218 / 225

页数：8

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