Functional analysis of expressed sequence tags from the liver and brain of Korean Jindo dogs

被引：1

作者：

Kim, Jae Young ^{[1
,3
]}

Park, Hye Sun ^{[1
]}

Lim, Dajeong ^{[1
]}

Jang, Hong Chul ^{[1
]}

Park, Hae Suk ^{[1
]}

Lee, Kyung Tai ^{[1
]}

Kim, Jong Seok ^{[2
]}

Oh, Seok Il ^{[2
]}

Kweon, Mu Sik ^{[3
]}

Kim, Tae Hun ^{[1
]}

Choi, Bong Hwan ^{[1
]}

机构：

[1] Rural Dev Adm, Natl Inst Anim Sci, Div Anim Genom & Bioinformat, Suwon 441706, South Korea

[2] Korean Jindo & Domest Anim Ctr, Jindo 539800, South Korea

[3] Sungkyunkwan Univ, Dept Genet Engn, Suwon 440746, South Korea

来源：

BMB REPORTS | 2011年 / 44卷 / 04期

关键词：

Brain; EST; Korean Jindo dog; Liver; Single Nucleotide Polymorphisms; CANINE; POLYMORPHISMS; MUTATIONS; ALIGNMENT; DATABASE; BLAST; SNPS;

D O I：

10.5483/BMBRep.2011.44.4.238

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We generated 16,993 expressed sequence tags (ESTs) from two libraries containing full-length cDNAs from the brain and liver of the Korean undo dog. An additional 365,909 ESTs from other dog breeds were identified from the NCBI dbEST database, and all ESTs were clustered into 28,514 consensus sequences using StackPack. We selected the 7,305 consensus sequences that could be assembled from at least five ESTs and estimated that 12,533 high-quality single nucleotide polymorphisms (SNPs) were present in 97,835 putative SNPs from the 7,305 consensus sequences. We identified 58 undo dog-specific SNPs in comparison to other breeds and predicted seven synonymous SNPs and ten non-synonymous SNPs. Using PolyPhen, a program that predicts changes in protein structure and potential effects on protein function caused by amino acid substitutions, three of the non-synonymous SNPs were predicted to result in changes in protein function for proteins expressed by three different genes (TUSC3, ITIH2, and NAT2). [BMB reports 2011; 44(4): 238-243]

引用

页码：238 / 243

页数：6

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