Antibody-based multiplex analysis of structurally closely related chiral molecules

被引：8

作者：

Kassa, Tigabu ^{[1
]}

Undesser, L. Pauli ^{[2
]}

Hofstetter, Heike ^{[1
]}

Hofstetter, Oliver ^{[1
]}

机构：

[1] No Illinois Univ, Dept Chem & Biochem, De Kalb, IL 60115 USA

[2] Pierce Biotechnol Thermo Fisher Sci, Rockford, IL 61105 USA

来源：

ANALYST | 2011年 / 136卷 / 06期

关键词：

PROTEIN MICROARRAYS; IMMUNOASSAY; SEPARATION; ACIDS;

D O I：

10.1039/c0an00814a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Four class-specific and stereoselective antibodies were labeled with different fluorophores to enable simultaneous quantification of the enantiomers of phenylalanine and phenyllactic acid. Using fluorescence immunoassays and microarrays, sensitive detection of each analyte was possible even in the presence of a large excess of the other structurally similar molecules.

引用

页码：1113 / 1115

页数：3

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