Dual-fluorophore ratiometric pH nanosensor with tuneable pKa and extended dynamic range

被引：48
作者：
Chauhan, Veeren M. [1 ]
Burnett, Gary R. [2 ]
Aylott, Jonathan W. [1 ]
机构：
[1] Sch Pharm, Lab Biophys & Surface Anal, Nottingham NG7 2RD, England
[2] GlaxoSmithKline Consumer Hlth, Weybridge KT13 0DE, Surrey, England
来源：
ANALYST | 2011年 / 136卷 / 09期
基金：
英国生物技术与生命科学研究理事会;
关键词：
LOCALIZED EMBEDDING PEBBLES; SINGLE LIVING CELLS; INTRACELLULAR PH; OPTICAL NANOSENSORS; CHEMICAL-ANALYSIS; LIVE CELLS; SENSORS; NANOPARTICLES; ELECTRODES; APOPTOSIS;
D O I：
10.1039/c1an15042a
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Ratiometric pH nanosensors with tuneable pK(a) were prepared by entrapping combinations of two pH-sensitive fluorophores (fluorescein isothiocyanate dextran (FITC-D) and Oregon Green (R) dextran (OG-D)) and a reference fluorophore (5-(and-6)-carboxyte-tramethylrhodamine dextran (TAMRA-D)), in a biocompatible polymer matrix. Dual-fluorophore pH nanosensors permit the measurement of an extended dynamic range, from pH 4.0 to 7.5.
引用
收藏
页码：1799 / 1801
页数：3
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