Dual-fluorophore ratiometric pH nanosensor with tuneable pKa and extended dynamic range

被引：49

作者：

Chauhan, Veeren M. ^{[1
]}

Burnett, Gary R. ^{[2
]}

Aylott, Jonathan W. ^{[1
]}

机构：

[1] Sch Pharm, Lab Biophys & Surface Anal, Nottingham NG7 2RD, England

[2] GlaxoSmithKline Consumer Hlth, Weybridge KT13 0DE, Surrey, England

来源：

ANALYST | 2011年 / 136卷 / 09期

基金：

英国生物技术与生命科学研究理事会;

关键词：

LOCALIZED EMBEDDING PEBBLES; SINGLE LIVING CELLS; INTRACELLULAR PH; OPTICAL NANOSENSORS; CHEMICAL-ANALYSIS; LIVE CELLS; SENSORS; NANOPARTICLES; ELECTRODES; APOPTOSIS;

D O I：

10.1039/c1an15042a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ratiometric pH nanosensors with tuneable pK(a) were prepared by entrapping combinations of two pH-sensitive fluorophores (fluorescein isothiocyanate dextran (FITC-D) and Oregon Green (R) dextran (OG-D)) and a reference fluorophore (5-(and-6)-carboxyte-tramethylrhodamine dextran (TAMRA-D)), in a biocompatible polymer matrix. Dual-fluorophore pH nanosensors permit the measurement of an extended dynamic range, from pH 4.0 to 7.5.

引用

页码：1799 / 1801

页数：3

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