Over-expression of the Arabidopsis Na+/H+ antiporter gene in Populus deltoides CL x P. euramericana CL "NL895" enhances its salt tolerance

被引:7
作者
Qiao, Guirong [1 ]
Zhuo, Renying [1 ]
Liu, Mingying [1 ]
Jiang, Jing [1 ]
Li, Haiying [1 ]
Qiu, Wenmin [1 ]
Pan, Luanyin [1 ]
Lin, Sheng [1 ,3 ]
Zhang, Xiaoguo [1 ]
Sun, Zongxiu [2 ]
机构
[1] Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Genom, Fuyang 311400, Zhejiang, Peoples R China
[2] China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
[3] Beijing Univ Chem Technol, Beijing 100029, Peoples R China
基金
中国国家自然科学基金;
关键词
AtNHX1; Populous; Mannose selection system; Salt stress; PHOSPHOMANNOSE ISOMERASE GENE; SELECTABLE MARKER; SELECTION SYSTEM; BIOLISTIC TRANSFORMATION; TRANSGENIC PLANTS; MANNOSE; TOMATO; L; AGROBACTERIUM; TOBACCO;
D O I
10.1007/s11738-010-0591-x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Soil salinity is a serious problem worldwide. It is necessary to improve the salt tolerance of plants to avoid the progressive deterioration of saline soil. We showed that the over-expression of AtNHX1 improves salt tolerance in a transgenic poplar (Populus deltoides CL x P. euramericana CL "NL895") under mannose selection. Four transgenic poplar plants were obtained. Southern blot analysis showed that the pmi gene had integrated into the genome of the poplar. RT-PCR confirmed that AtNHX1 could be expressed normally in the transgenic plants. When tested for salt tolerance by NaCl stress, we measured a 100% increase in Na+ content in the three transgenic lines (T18, T50, T98) significantly higher than the 33% increase seen in wild-type plants. The chlorophyll content of the transgenic plants was not altered significantly, while the chlorophyll content in the control plants showed a small decrease. MDA content was decreased in the transgenic plants. These results show that the AtNHX1 gene may enhance salt tolerance due to increased vacuolar compartmentalization of sodium ions.
引用
收藏
页码:691 / 696
页数:6
相关论文
共 28 条
  • [1] Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis
    Apse, MP
    Aharon, GS
    Snedden, WA
    Blumwald, E
    [J]. SCIENCE, 1999, 285 (5431) : 1256 - 1258
  • [2] Agrobacterium and biolistic transformation of onion using non-antibiotic selection marker phosphomannose isomerase
    Aswath, CR
    Mo, SY
    Kim, DH
    Park, SW
    [J]. PLANT CELL REPORTS, 2006, 25 (02) : 92 - 99
  • [3] A Nicotiana tabacum cell culture selected for accelerated growth on mannose has increased expression of phosphomannose isomerase
    Barb, AW
    Pharr, DM
    Williamson, JD
    [J]. PLANT SCIENCE, 2003, 165 (03) : 639 - 648
  • [4] The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)
    Boscariol, RL
    Almeida, WAB
    Derbyshire, MTVC
    Mourao, FAA
    Mendes, BMJ
    [J]. PLANT CELL REPORTS, 2003, 22 (02) : 122 - 128
  • [5] Doyle JJ, 1987, PHYTOCHEM B, V19, P11, DOI DOI 10.2307/4119796
  • [6] The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast
    Gaxiola, RA
    Rao, R
    Sherman, A
    Grisafi, P
    Alper, SL
    Fink, GR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (04) : 1480 - 1485
  • [7] CARBOHYDRATE NUTRITION OF TOMATO ROOTS .8. MECHANISM OF INHIBITION BY D-MANNOSE OF RESPIRATION OF EXCISED ROOTS
    GOLDSWORTHY, A
    STREET, HE
    [J]. ANNALS OF BOTANY, 1965, 29 (113) : 45 - +
  • [8] Mannose selection system used for cucumber transformation
    He, Zhengquan
    Duan, ZhenZhen
    Liang, Wei
    Chen, Faju
    Yao, Wei
    Liang, Hongwei
    Yue, Chaoyin
    Sun, Zongxiu
    Chen, Fan
    Dai, Jianwu
    [J]. PLANT CELL REPORTS, 2006, 25 (09) : 953 - 958
  • [9] HOLSTER SM, 1978, MOL GEN GENET, V163, P181
  • [10] NEW AGROBACTERIUM HELPER PLASMIDS FOR GENE-TRANSFER TO PLANTS
    HOOD, EE
    GELVIN, SB
    MELCHERS, LS
    HOEKEMA, A
    [J]. TRANSGENIC RESEARCH, 1993, 2 (04) : 208 - 218