Synthesis of deuterated isotopomers of 7α- and (25R,S)-26-hydroxycholesterol, internal standards for in vivo determination of the two biosynthetic pathways of bile acids

被引：10

作者：

Ciuffreda, P ^{[1
]}

Casati, S ^{[1
]}

Alessandrini, L ^{[1
]}

Terraneo, G ^{[1
]}

Santaniello, E ^{[1
]}

机构：

[1] Univ Milan, Dipartimento Sci Preclin LITA Vialba, I-20157 Milan, Italy

来源：

STEROIDS | 2003年 / 68卷 / 09期

关键词：

deuterated isotopomers; 7; alpha-hydroxycholesterol; (25R)-26-hydroxycholesterol; bile acids; cholesterol metabolism;

D O I：

10.1016/S0039-128X(03)00116-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Deuterated isotopomers of 7alpha- and (25R,S)-26-hydroxycholesterol, internal standards for in vivo determination of the two biosynthetic pathways of bile acids formation from cholesterol, were prepared from [2,2,3,4,4,6-H-2(6)]-cholesterol and (20S)-[7,7,21,21-H-2(4)]-3beta-(tert-butyldimethylsilyl)oxy-20-methylpregna-5-en-21-ol, respectively. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：733 / 738

页数：6

共 15 条

[1]

BJOERKHEM I, 1992, J LIPID RES, V33, P455

[2] Synthesis of (20S)-[7,7,21,21-2H4]-3β-(tert-butyldimethylsilanyloxy)-20-methyl-pregn-5-en-21-ol, a useful intermediate for the preparation of deuterated isotopomers of sterols [J].