An easy-to-use tool for planning and modeling a calorimetric titration

被引：12

作者：

Biswas, Tapan ^{[1
]}

Tsodikov, Oleg V. ^{[1
]}

机构：

[1] Univ Michigan, Coll Pharm, Dept Med Chem, Ann Arbor, MI 48109 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 406卷 / 01期

关键词：

ESCHERICHIA-COLI; DNA-BINDING; COMPLEX;

D O I：

10.1016/j.ab.2010.06.050

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Isothermal titration calorimetry (ITC) is widely employed to measure thermodynamic properties of binding interactions between two macromolecules or a macromolecule and a small ligand. No labeling of interacting species is required for ITC, but this advantage is offset by potentially material-consuming experimental optimization complicated by an indirect readout of an ITC titration. Here we present a simple, practical, and portable spreadsheet-based tool for planning and modeling an ITC titration experiment accompanied by basic guidelines. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：91 / 93

页数：3