Dynamic behavior of FCHO1 revealed by live-cell imaging microscopy: Its possible involvement in clathrin-coated vesicle formation

被引：15

作者：

Sakaushi, Shinji

Inoue, Kimiko

Zushi, Hitomi

Senda-Murata, Kaori

Fukada, Takashi

Oka, Shigenori

Sugimoto, Kenji

机构：

[1] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Div Biosci & Informat, Lab Mol Biol & Cell Informat,Naka Ku, Sakai, Osaka 5998531, Japan

[2] Osaka Prefecture Univ, Lab Appl Mol Biol, Div Appl Biochem, Grad Sch Agr & Biol Sci,Naka Ku, Sakai, Osaka 5998531, Japan

[3] Nagase & Co Ltd, Ctr Res & Dev, Labs 2, Nishi Ku, Kobe, Hyogo 6512241, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2007年 / 71卷 / 07期

关键词：

FCHO1; KIAA0290; clathrin; periodic fluctuations (PFs); dual-color imaging;

D O I：

10.1271/bbb.60720

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The intracellular behavior of human FCHO1 protein was investigated by live-cell imaging microscopy. The fluorescence intensity of green fluorescent protein (GFP)-FCHO1 fluctuated periodically in a perinuclear region approximately every 100 s, reminding us of the periodic fluctuations of clathrin reported in our recent work. The periodicity of FCHO1 was temporally correlated with that of clathrin, suggesting that FCHO1 is involved in clathrin-coated vesicle formation.

引用

页码：1764 / 1768

页数：5