HPLC Separation of Acetaminophen and its Impurities Using A Mixed-mode Reversed-Phase/Cation Exchange Stationary Phase

被引:46
作者
Calinescu, Octavian [1 ]
Badea, Irinel A. [1 ]
Vladescu, Luminita [1 ]
Meltzer, Viorica [2 ]
Pincu, Elena [2 ]
机构
[1] Univ Bucharest, Fac Chem, Dept Analyt Chem, Bucharest, Romania
[2] Univ Bucharest, Fac Chem, Dept Phys Chem, Bucharest, Romania
关键词
P-AMINOPHENOL; LIQUID-CHROMATOGRAPHY; SPECTROPHOTOMETRIC DETERMINATION; CAPILLARY ELECTROCHROMATOGRAPHY; PARACETAMOL; 4-AMINOPHENOL; DEGRADATION; RETENTION; TEMPERATURE; ARRAY;
D O I
10.1093/chromsci/bmr043
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Determination of acetaminophen and its main impurities: 4-nitrophenol, 4'-chloroacetanilide, as well as 4-aminophenol and its degradation products, p-benzoquinone and hydroquinone has been developed and validated by a new high-performance liquid chromatography method. Chromatographic separation has been obtained on a Hypersil Duet C18/SCX column, using gradient elution, with a mixture of phosphate buffer (pH=4.88) and methanol as a mobile phase. Analysis time did not exceed 14.5 min and good resolutions, peak shapes and asymmetries have resulted. The linearity of the method has been tested in the range of 5.0-60 μg/mL for acetaminophen and 0.56 μg/mL for the other compounds. The limits of detection and quantification have been also established to be lower than 0.1 μg/mL and 0.5 μg/mL, respectively. The method has been successfully applied for the analysis of commercial acetaminophen preparations. © 2012 The Author.
引用
收藏
页码:335 / 342
页数:8
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