HPLC Separation of Acetaminophen and its Impurities Using A Mixed-mode Reversed-Phase/Cation Exchange Stationary Phase

被引：46

作者：

Calinescu, Octavian ^{[1
]}

Badea, Irinel A. ^{[1
]}

Vladescu, Luminita ^{[1
]}

Meltzer, Viorica ^{[2
]}

Pincu, Elena ^{[2
]}

机构：

[1] Univ Bucharest, Fac Chem, Dept Analyt Chem, Bucharest, Romania

[2] Univ Bucharest, Fac Chem, Dept Phys Chem, Bucharest, Romania

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2012年 / 50卷 / 04期

关键词：

P-AMINOPHENOL; LIQUID-CHROMATOGRAPHY; SPECTROPHOTOMETRIC DETERMINATION; CAPILLARY ELECTROCHROMATOGRAPHY; PARACETAMOL; 4-AMINOPHENOL; DEGRADATION; RETENTION; TEMPERATURE; ARRAY;

D O I：

10.1093/chromsci/bmr043

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Determination of acetaminophen and its main impurities: 4-nitrophenol, 4'-chloroacetanilide, as well as 4-aminophenol and its degradation products, p-benzoquinone and hydroquinone has been developed and validated by a new high-performance liquid chromatography method. Chromatographic separation has been obtained on a Hypersil Duet C18/SCX column, using gradient elution, with a mixture of phosphate buffer (pH=4.88) and methanol as a mobile phase. Analysis time did not exceed 14.5 min and good resolutions, peak shapes and asymmetries have resulted. The linearity of the method has been tested in the range of 5.0-60 μg/mL for acetaminophen and 0.56 μg/mL for the other compounds. The limits of detection and quantification have been also established to be lower than 0.1 μg/mL and 0.5 μg/mL, respectively. The method has been successfully applied for the analysis of commercial acetaminophen preparations. © 2012 The Author.

引用

页码：335 / 342

页数：8

共 35 条

[1]

Ali MS, 2007, J AOAC INT, V90, P82

[2]

[Anonymous], CPMPICH38195

[3]

[Anonymous], 2007, British Pharmacopoeia, VII

[4] DEVELOPMENT OF A NEW HPLC METHOD FOR DETERMINATION OF PAPAVERINE IN PRESENCE OF ITS PHOTOOXIDATION PRODUCTS [J].

Badea, Irinel A. ;

Vladescu, Luminita ;

David, Iulia G. ;

David, Vasile ;

Litescu, Simona C. .

ANALYTICAL LETTERS, 2010, 43 (7-8) :1217-1229

[5] A sensitive and rapid assay for 4-aminophenol in paracetamol drug and tablet formulation, by flow injection analysis with spectrophotometric detection [J].

Bloomfield, MS .

TALANTA, 2002, 58 (06) :1301-1310

[6] Determination of paracetamol:: Historical evolution [J].

Bosch, M. Espinosa ;

Sanchez, A. J. Ruiz ;

Rojas, F. Sanchez ;

Ojeda, C. Bosch .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2006, 42 (03) :291-321

[7] Retention of ionizable compounds on HPLC.: Modelling retention in reversed-phase liquid chromatography as a function of pH and solvent composition with methanol-water mobile phases [J].

Canals, I ;

Portal, JA ;

Rosés, M ;

Bosch, E .

CHROMATOGRAPHIA, 2002, 55 (9-10) :565-571

[8] DETERMINATION OF GRADIENT ELUTION CONDITIONS FOR THE SEPARATION OF PEPTIDE MIXTURES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - BOVINE BETA-CASEIN TRYPTIC DIGEST [J].

CARLES, C ;

RIBADEAUDUMAS, B .

JOURNAL OF DAIRY RESEARCH, 1986, 53 (04) :595-600

[9] High-performance liquid chromatographic separation of rolipram, bupivacaine and omeprazole using a tartardiamide-based stationary phase Influence of flow rate and temperature on the enantioseparation [J].

da Silva, Ivanildo Jose Junior ;

Sartor, Joao Paulo ;

Pires Rosa, Paulo Cesar ;

de Veredas, Vinicius ;

Barreto, Amaro Gomes Junior ;

Santana, Cesar Costapinto .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1162 (01) :97-102

[10] A study of retention and overloading of basic compounds with mixed-mode reversed-phase/cation-exchange columns in high performance liquid chromatography [J].

Davies, Nicola H. ;

Euerby, Melvin R. ;

McCalley, David V. .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1138 (1-2) :65-72

← 1 2 3 4 →