Peptide-bond synthesis on the ribosome: No free vicinal hydroxy group required on the terminal ribose residue of peptidyl-tRNA

被引：31

作者：

Koch, Miriam ^{[1
]}

Huang, Yiwei ^{[1
]}

Sprinzl, Mathias ^{[1
]}

机构：

[1] Univ Bayreuth, Biochem Lab, D-95440 Bayreuth, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 38期

关键词：

peptides; reaction mechanisms; ribozymes; transferases; tRNA;

D O I：

10.1002/anie.200801511

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) OH? - OH no! The generally accepted explanation for peptide transfer in ribosomal peptidyltransferase is that the 2′-OH group of the adenosine residue (Ad) in the P site accepts a proton from the incoming α-amino group and transfers it to the outgoing deacylated tRNA (left). However, it has now been shown that peptide transfer still takes place in the absence of the 2′-hydroxy group (right: proposed alternative mechanism). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：7242 / 7245

页数：4

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