Biomagnetic glass beads for protein separation and detection based on surface-enhanced Raman scattering

被引：12

作者：

Chen, Lei ^{[1
]}

Han, Xiao Xia ^{[1
]}

Guo, Zhinan ^{[1
]}

Wang, Xu ^{[1
]}

Ruan, Weidong ^{[1
]}

Song, Wei ^{[1
]}

Zhao, Bing ^{[1
]}

Ozaki, Yukihiro ^{[2
,3
]}

机构：

[1] Jilin Univ, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China

[2] Kwansei Gakuin Univ, Sch Sci & Technol, Dept Chem, Sanda, Hyogo 6691337, Japan

[3] Kwansei Gakuin Univ, Sch Sci & Technol, Res Ctr Single Mol Spect, Sanda, Hyogo 6691337, Japan

来源：

ANALYTICAL METHODS | 2012年 / 4卷 / 06期

关键词：

IRON-OXIDE NANOPARTICLES; MAGNETIC NANOPARTICLES; LABEL; IMMOBILIZATION; MICROSPHERES; SPECTROSCOPY; TRYPSIN; DESIGN; SILVER; SHELL;

D O I：

10.1039/c2ay25244a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel surface-enhanced Raman scattering (SERS)-based detection method for proteins attached to magnetic glass beads have been developed in this study. Silica coated magnetic nanoparticles (Fe3O4) are prepared and aldehyde-functionalized for capturing proteins in solutions by Schiff reaction, which is more biocompatible than previous magnetic nanoparticle and SERS-based detection methods. Target protein and molecule with a specially recognized feature are captured and separated by the magnetic nanoparticles, followed by SERS-based detection. Moreover, these biomagnetic glass beads and detection protocol are found to be useful in protein immunoassay and protein-ligand recognition even in a mixture system (two protein ligands). The proposed magnetic nanoparticles are biocompatible, manipulatable by an external magnetic force and highly sensitive after silver nanoparticles staining, which makes the protocol quite promising for high throughput protein assays.

引用

页码：1643 / 1647

页数：5

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