Evaluation of the response of ruminal fermentation and activities of nonstarch polysaccharide-degrading enzymes to particle length of corn silage in dairy cows

The main objective of this study was to evaluate effects of particle length (PL) of corn silage (CS) on distribution of dietary particle fractions, contents of physically effective neutral detergent fiber (peNDF), cows' intake patterns and sorting activity, fermentation profile, and activities of nonstarch polysaccharide-degrading enzymes as well as degradation in the rumen and total tract in lactating dairy cows. Four ruminally cannulated Holstein cows, weighing 624 +/- 50 kg and 60 +/- 8 d in milk, were fed ad libitum 3 total mixed rations [about 16% crude protein, 34% neutral detergent fiber (NDF), and 7 MJ of net energy of lactation/kg of dry matter (DM)] containing on DM basis 50% concentrate, 10% grass hay, and 40% CS with 3 different theoretical PL at harvesting (14, 8.1, and 5.5 mm for long, medium, and short, respectively). Results showed that the amount of DM retained on sieves with 19- and 8-mm screens of Penn State Particle Separator decreased linearly with decreasing PL of CS. The latter was reflected in a significant decrease in the content of dietary peNDF including both the DM (peNDF(>8)) and the NDF (peNDF(>8-NDF)) retained on 19- and 8-mm screens. In contrast, the fraction of particles retained between the 1.18- and 8-mm screens was increased, such that no differences among the diets were observed regarding the content of peNDF that includes DM of particles > 1.18mm(peNDF(>1.18)). The intake of particles retained between the 1.18- and 8-mm screens increased linearly, whereas the intake of peNDF(>1.18) increased quadratically with decreasing PL of CS. Sorting consumption was reduced by feeding the short CS, which was reflected in a reduced proportion of propionate and increased acetate-to-propionate ratio and butyrate proportion in the rumen. In contrast, no effects of PL of CS were observed on the concentration of total volatile fatty acids and pH in the rumen. In general, decreasing the PL of CS significantly increased the activities of nonstarch polysaccharide-degrading enzymes. However, greater ruminal and total tract degradation of fiber and nonfiber carbohydrates were observed only by medium CS. Results of the present study suggest that in addition to fractions of long particles (i.e., > 8 mm), the particle fraction retained between 1.18- and 8-mm should also be considered to better predict rumen conditions and digestion. In conclusion, a moderate reduction of PL of CS has beneficial effects on nutrient digestion, and may maximize feed efficiency and energy supply in high-yielding dairy cows.