Assessment of a fully automated RPR assay (Mediace RPR) for serological diagnosis and follow-up of syphilis: a retrospective study

被引：3

作者：

Leroy, Anne-Gaelle ^{[1
]}

Robert, Manon ^{[1
]}

Carpentier, Maxime ^{[1
]}

Bastidon, Christophe ^{[2
]}

Gautreau, Blandine ^{[1
]}

Lefebvre, Maeva ^{[3
]}

Bonnet, Benedicte ^{[4
]}

Bernier, Claire ^{[4
]}

Corveca, Stephane ^{[1
,5
]}

Guillouzouica, Aurelie ^{[1
]}

机构：

[1] CHU Nantes Univ Hosp, Dept Microbiol, Nantes, France

[2] CHU Nantes Univ Hosp, Biochem Lab, Nantes, France

[3] CHU Nantes Hotel Dieu, Dept Infect Dis, Nantes, France

[4] CHU Nantes Univ Hosp, Ctr Prevent Infect & Transmissible Dis, Nantes, France

[5] Univ Nantes, INSERM, Nantes, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2022年 / 104卷 / 03期

关键词：

Syphilis; Automated non-treponemal test; Rapid plasma reagin; Syphilis follow-up; Treponema pallidum; LABORATORY DIAGNOSIS;

D O I：

10.1016/j.diagmicrobio.2022.115767

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: This study assessed the Mediace RPR assay, an automated RPR (aRPR), for syphilis diagnosis and serological follow-up.Methods: Serums from patients positively screened for syphilis between January 2017 and December 2019 were retrospectively selected. A focus was performed on patients with a serological follow-up after treat-ment and/or a reinfection. Serums were tested by both manual (mRPR) and aRPR tests. Categorical and Quantitative Agreements (CA and QA), and serological follow-up conclusions were analyzed.Results: 236 serums from 85 patients (99% of male, 66% of HIV-infected) were included. The overall QA was 54.2%. CA was low (79.7%) especially for samples with low RPR titers. No prozone effect was observed. Sero-logical follow-up after treatment led to similar conclusions, although aRPR titers often decreased faster. Over 26 episodes of reinfection, 4 (15.4%) were misdiagnosed with the aRPR.Conclusions: While the Mediace aRPR presents the advantages of an automated test, its poor sensitivity in low titers may limit its use. (c) 2022 Elsevier Inc. All rights reserved.

引用

页数：5

共 13 条

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