Response of Scenedesmus quadricauda (Chlorophyceae) to Salt Stress Considering Nutrient Enrichment and Intracellular Proline Accumulation

被引:19
作者
Park, Myung-Hwan [1 ]
Park, Chae-Hong [2 ]
Sim, Yeon Bo [3 ]
Hwang, Soon-Jin [2 ,3 ]
机构
[1] Hanyang Univ, Res Inst Nat Sci, Seoul 04763, South Korea
[2] Konkuk Univ, Human & Ecocare Ctr, Seoul 05029, South Korea
[3] Konkuk Univ, Dept Environm Hlth Sci, Seoul 05029, South Korea
关键词
N and P enrichment; eutrophication; survival; growth; green algae; N:P ratio; LIPID-PEROXIDATION; ANABAENA-DOLIOLUM; SALINITY STRESS; GREEN-ALGAE; GROWTH; WATER; BIOMASS; LAKE; BIOSYNTHESIS; METABOLISM;
D O I
10.3390/ijerph17103624
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Aquatic organisms are exposed to a wide range of salinity, which could critically affect their survival and growth. However, their survival and growth response to salinity stress remain unclear. This study evaluates the growth response and intracellular proline accumulation of green algae, Scenedesmus quadricauda, isolated from brackish water, against dissolved salts stress with N and P enrichment. We tested a hypothesis that nutrient enrichment can relieve the dissolved salts stress of algae by accumulating intracellular proline, thereby improving survival and growth. Four levels of salinity (0, 3, 6, 12 psu) were experimentally manipulated with four levels of nutrient stoichiometry (N:P ratio = 2, 5, 10, 20) at constant N (1 mgN/L) or P levels (0.05 and 0.5 mgP/L). In each set of experiments, growth rate and intracellular proline content were measured in triplicate. The highest level of salinity inhibited the growth rate of S. quadricauda, regardless of the nutrient levels. However, with nutrient enrichment, the alga showed tolerance to dissolved salts, reflecting intracellular proline synthesis. Proline accumulation was most prominent at the highest salinity level, and its maximum value appeared at the highest N:P ratio (i.e., highest N level) in all salinity treatments, regardless of P levels. Therefore, the effects of P and N on algal response to salt stress differ.
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页数:12
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