Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells

被引：39

作者：

Fijak, Monika ^{[1
]}

Damm, Lara-Jil ^{[1
]}

Wenzel, Jan-Per ^{[1
]}

Aslani, Ferial ^{[1
]}

Walecki, Magdalena ^{[1
]}

Wahle, Eva ^{[1
]}

Eisel, Florian ^{[1
]}

Bhushan, Sudhanshu ^{[1
]}

Hackstein, Holger ^{[2
]}

Baal, Nelli ^{[2
]}

Schuler, Gerhard ^{[3
]}

Konrad, Lutz ^{[4
]}

Rafiq, Amir ^{[1
]}

O'Hara, Laura ^{[5
]}

Smith, Lee B. ^{[5
]}

Meinhardt, Andreas ^{[1
]}

机构：

[1] Univ Giessen, Dept Anat & Cell Biol, D-35392 Giessen, Germany

[2] Univ Giessen, Inst Clin Immunol & Transfus Med, D-35392 Giessen, Germany

[3] Univ Giessen, Fac Vet Med, Clin Obstet Gynecol & Androl Large & Small Anim, D-35392 Giessen, Germany

[4] Univ Giessen, Fac Med, Dept Obstet & Gynaecol, D-35392 Giessen, Germany

[5] Univ Edinburgh, MRC Ctr Reprod Hlth, Edinburgh, Midlothian, Scotland

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2015年 / 74卷 / 01期

关键词：

Foxp3; peritubular cells; sertoli cells; testicular immune response; testicular macrophages; testosterone; TUMOR-NECROSIS-FACTOR; HUMAN CHORIONIC-GONADOTROPIN; RECEPTOR-FREE MACROPHAGES; ANDROGEN RECEPTOR; IMMUNE PRIVILEGE; SERTOLI-CELLS; LEYDIG-CELLS; FACTOR-ALPHA; AUTOIMMUNE ORCHITIS; ENDOTHELIAL-CELLS;

D O I：

10.1111/aji.12363

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

ProblemPrevious studies demonstrated a strong association between low androgen levels and reduced capacity to mount an inflammatory response. However, the mechanisms underlying these observations are largely not understood. Methods of studyGeneration of CD4+CD25+Foxp3+ regulatory T cells in Leydig cell-conditioned media was determined by flow cytometry and ELISA. Influence of testosterone on cytokine response was measured in LPS-stimulated testicular macrophages, Sertoli and peritubular cells. ResultsLeydig cell-conditioned media dose-dependently stimulated expression of transcription factor Foxp3 and secretion of IL-10 in splenic CD4+ T cells, an effect abolished by addition of the anti-androgen flutamide. In isolated Sertoli and peritubular cells, testosterone pre-treatment suppressed the LPS-induced inflammatory response on TNF- mRNA expression, while no effect was evident in testicular macrophages (TM). ConclusionsAndrogens can influence the immune system under normal conditions by the generation and functional differentiation of regulatory T cells and in testicular inflammation by direct effect on Sertoli and peritubular cells.

引用

页码：12 / 25

页数：14

共 78 条

[1] Cytokines and Chemokines in Testicular Inflammation: A Brief Review [J].