The Optimization of a Lateral Flow Immunoassay for Detection of Aflatoxin B1 in Potable Water Samples

被引：16

作者：

Yan, Xiaoxiang ^{[1
,2
]}

Persaud, Krishna C. ^{[1
]}

机构：

[1] Univ Manchester, Sch Chem Engn & Analyt Sci, Manchester M13 9PL, Lancs, England

[2] Univ Glasgow, Glasgow G12 8QQ, Lanark, Scotland

来源：

IEEE SENSORS JOURNAL | 2019年 / 19卷 / 02期

关键词：

Aflatoxin B-1; biosensor; lateral flow immunoassay; potable water; IMMUNOCHROMATOGRAPHIC ASSAY; LIQUID; SENSITIVITY;

D O I：

10.1109/JSEN.2018.2878449

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Aflatoxin B-1 (AFB(1)) is a highly toxic and carcinogenic metabolite produced by fungi of genus Aspergillus. While AFB(1) contamination in food and crops is well known, little attention has been paid to potable water which is another potential source of AFB(1) contamination that can be ingested by humans. In order to develop a robust, rapidly manufactured sensing device that can monitor the AFB(1) contamination in water, a lateral flow immunostrip based on gold nanoparticles (AuNPs) has been optimized for determination of AFB(1) in potable water samples. A visual detection limit (LOD) of 0.5 ppb was achieved for determination of AFB(1) for the majority of water samples tested. The strip remained functional after being stored at 4 degrees C for 3 months.

引用

页码：404 / 412

页数：9