Molecular and Catalytic Properties of 2,4-Diacetylphloroglucinol Hydrolase (PhlG) from Pseudomonas sp YGJ3

被引：3
作者：
Saitou, Hiroki [1 ]
Watanabe, Manami [1 ]
Maruyama, Kiyofumi [1 ]
机构：
[1] Gifu Univ, Dept Biomol Sci, Fac Engn, Gifu 5011193, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2012年 / 76卷 / 06期
关键词：
biocontrol; diacetylphloroglucinol; hydrolase; PhlG; FLUORESCENS CHA0; BIOSYNTHESIS; STRAINS;
D O I：
10.1271/bbb.120054
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Gene phlG encoding 2,4-diacetylphloroglucinol hydrolase was cloned from Pseudomonas sp. YGJ3 and expressed in Escherichia coli. Recombinant PhlG was purified homogeneously. It required 2-mercaptoethanol for stability. K-m for 2,4-diacetylphloroglucinol and k(cat) were determined to be 24 mu M and 5.8 s(-1) respectively. CoCl2 specifically and significantly activated PhlG.
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收藏
页码：1239 / 1241
页数：3
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