Isolation of yeast candidates for efficient sophorolipids production: their production potentials associate to their lineage

被引：12

作者：

Konishi, Masaaki ^{[1
]}

Fujita, Manako ^{[1
]}

Ishibane, Yu ^{[1
]}

Shimizu, Yuki ^{[1
]}

Tsukiyama, Yusuke ^{[1
]}

Ishida, Masashi ^{[1
]}

机构：

[1] Kitami Inst Technol, Dept Biotechnol & Environm Chem, Kitami, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2016年 / 80卷 / 10期

基金：

日本学术振兴会;

关键词：

biosurfactant; Candida floricola; sophorolipids; Starmerella bombicola; yeast; BOMBICOLA; BIOSURFACTANTS; GLYCOLIPIDS;

D O I：

10.1080/09168451.2016.1191332

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eleven biosurfactant-producing strains were newly isolated from environmental samples using a drop-collapse assay and thin-layer chromatography (TLC). According to the TLC analysis, the separation patterns of the glycolipid spots of nine dominant strains corresponded to that of the sophorolipids produced by a Starmerella bombicola type strain. The retention factor values of the spot patterns of two strains were less than those of the others. Two representative major products were purified, and their molecular structures were determined. The major products were identified as diacetylated lactonic and acidic sophorolipids. The fatty acid moieties of both compounds were estimated to be 17-hydroxymethyl hexadecenoic acid. The amounts of glycolipids ranged from 5.0 to 22.9g/L after 4 d of cultivation. According to a phylogenetic analysis, the strains were identified as Starmerella bombicola and Candida floricola.

引用

页码：2058 / 2064

页数：7

共 25 条

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