HTR1A a Novel Type 1 Diabetes Susceptibility Gene on Chromosome 5p13-q13

被引:18
作者
Asad, Samina [1 ]
Nikamo, Pernilla [2 ]
Gyllenberg, Alexandra [1 ]
Bennet, Hedvig [3 ,4 ]
Hansson, Ola [4 ]
Wierup, Nils [4 ]
Carlsson, Annelie [5 ]
Forsander, Gun [6 ]
Ivarsson, Sten-Anders [3 ]
Larsson, Helena [3 ]
Lernmark, Ake [3 ]
Lindblad, Bengt [6 ]
Ludvigsson, Johnny [7 ]
Marcus, Claude [8 ]
Ronningen, Kjersti S. [9 ]
Nerup, Jan [10 ]
Pociot, Flemming [3 ,11 ]
Luthman, Holger [3 ,4 ]
Fex, Malin [3 ,4 ]
Kockum, Ingrid [1 ]
机构
[1] Karolinska Inst, Dept Clin Neurosci, Neuroimmunol Unit, Stockholm, Sweden
[2] Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden
[3] Lund Univ, Malmo Univ Hosp, Dept Clin Sci, Diabet & Celiac Unit, Malmo, Sweden
[4] Lund Univ, Ctr Diabet, Lund, Sweden
[5] Univ Lund Hosp, Dept Pediat, S-22185 Lund, Sweden
[6] Queen Silvia Childrens Hosp, Dept Pediat, Gothenburg, Sweden
[7] Linkoping Univ Hosp, Div Pediat, Dept Clin & Expt Med, Diabet Res Ctr, S-58185 Linkoping, Sweden
[8] Karolinska Inst, Div Pediat, Dept Clin Sci Intervent & Technol, Natl Childhood Obes Ctr, Stockholm, Sweden
[9] Univ Oslo, Rikshosp, Oslo Univ Hosp, Dept Pediat Res, N-0027 Oslo, Norway
[10] Steno Diabet Ctr, DK-2820 Gentofte, Denmark
[11] Univ Hosp Glostrup, Glostrup Res Inst, Glostrup, Denmark
基金
瑞典研究理事会;
关键词
SYSTEMIC-LUPUS-ERYTHEMATOSUS; ASSOCIATION ANALYSIS; RECEPTORS; LINKAGE; RISK; SEROTONIN; MULTIPLEX; FAMILIES; PEPTIDE; REGIONS;
D O I
10.1371/journal.pone.0035439
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: We have previously performed a genome-wide linkage study in Scandinavian Type 1 diabetes (T1D) families. In the Swedish families, we detected suggestive linkage (LOD <= 2.2) to the chromosome 5p13-q13 region. The aim of our study was to investigate the linked region in search for possible T1D susceptibility genes. Methodology/Principal Findings: Microsatellites were genotyped in the Scandinavian families to fine-map the previously linked region. Further, SNPs were genotyped in Swedish and Danish families as well as Swedish sporadic cases. In the Swedish families we detected genome-wide significant linkage to the 5-hydroxytryptamine receptor 1A (HTR1A) gene (LOD 3.98, p<9.8x10(-6)). Markers tagging two separate genes; the ring finger protein 180 (RNF180) and HTR1A showed association to T1D in the Swedish and Danish families (p<0.002, p<0.001 respectively). The association was not confirmed in sporadic cases. Conditional analysis indicates that the primary association was to HTR1A. Quantitative PCR show that transcripts of both HTR1A and RNF180 are present in human islets of Langerhans. Moreover, immunohistochemical analysis confirmed the presence of the 5-HTR1A protein in isolated human islets of Langerhans as well as in sections of human pancreas. Conclusions: We have identified and confirmed the association of both HTR1A and RFN180, two genes in high linkage disequilibrium (LD) to T1D in two separate family materials. As both HTR1A and RFN180 were expressed at the mRNA level and HTR1A as protein in human islets of Langerhans, we suggest that HTR1A may affect T1D susceptibility by modulating the initial autoimmune attack or either islet regeneration, insulin release, or both.
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页数:10
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