Quantitative determination of SH groups using 19F NMR spectroscopy and disulfide of 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid

被引：8

作者：

Potapenko, DI

Bagryanskaya, EG

Grigoriev, IA

Maksimov, AM

Reznikov, VA

Platonov, VE

Clanton, TL

Khramtsov, VV

机构：

[1] Ohio State Univ, Dorothy M Davis Heart & Lung Res Inst, Columbus, OH 43210 USA

[2] Int Tomog Ctr, Novosibirsk 630090, Russia

[3] Novosibirsk State Univ, Novosibirsk 630090, Russia

[4] Inst Organ Chem, Novosibirsk 630090, Russia

[5] Inst Chem Kinet & Combust, Novosibirsk 630090, Russia

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 2005年 / 43卷 / 11期

关键词：

NMR; thiols; thiol measurements; F-19; thiol-disulfide exchange; glutathione; fluorinated disulfides; benzoic acid;

D O I：

10.1002/mrc.1652

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new method of measurement of thiol concentration by F-19 NMR spectroscopy is developed. The method is based on the detection of products of the exchange reaction of thiols with a newly synthesized fluorinated disulfide, 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid (BSSB). A significant broadening of the F-19 NMR signal of BSSB in the presence of thiols was observed and attributed to the exchange reaction between the parent disulfide and 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid. The rate constant for this reaction was found to be equal to (63 +/- 11) X 10(3) M-1 s(-1) at pH 7.0. The method was applied for the measurement of concentration of glutathione and albumin in rat blood. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：902 / 909

页数：8

共 17 条

[1] Augmented water binding and low cellular water content in erythrocytes of camel and camelids [J].

Bogner, P ;

Csutora, P ;

Cameron, IL ;

Wheatley, DN ;

Miseta, A .

BIOPHYSICAL JOURNAL, 1998, 75 (06) :3085-3091

[2] METHODOLOGY FOR ANALYSIS OF TISSUE SULFHYDRYL COMPONENTS [J].

BOYNE, AF ;

ELLMAN, GL .

ANALYTICAL BIOCHEMISTRY, 1972, 46 (02) :639-&

[3]

Carrington A., 1979, INTRO MAGNETIC RESON

[4]

DEBRUIN SH, 1977, EUR J BIOCHEM, V75, P211

[5]

EMSLEY JW, 1966, HIGH RESOLUTION NUCL, V1

[6]

FIELDING HC, 1992, J FLUORINE CHEM, V39, P15

[7]

Jocelyn P.C., 1972, BIOCH SH GROUPS

[8]

JOCELYN PC, 1987, METHOD ENZYMOL, V143, P44

[9]

Khramtsov V, 1999, MAGNET RESON MED, V42, P228, DOI 10.1002/(SICI)1522-2594(199908)42:2<228::AID-MRM3>3.0.CO

[10]

2-T

← 1 2 →