Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions

被引：21

作者：

Sandhu, Nitika ^{[1
]}

Singh, Jasneet ^{[1
]}

Singh, Gursewak ^{[1
]}

Sethi, Mehak ^{[1
]}

Singh, Mohini Prabha ^{[1
]}

Pruthi, Gomsie ^{[1
]}

Raigar, Om Prakash ^{[1
]}

Kaur, Rupinder ^{[1
]}

Sarao, Preetinder Singh ^{[1
]}

Lore, Jagjeet Singh ^{[1
]}

Singh, Uma Maheshwar ^{[2
]}

Dixit, Shilpi ^{[2
]}

Sagare, Deepti ^{[3
]}

Singh, S. P. ^{[4
]}

Satturu, Vanisri ^{[5
]}

Singh, Vikas KUmar ^{[3
]}

Kumar, Arvind ^{[2
,6
]}

机构：

[1] Punjab Agr Univ, Ludhiana 141004, Punjab, India

[2] Int Rice Res Inst IRRI, South Asia Reg Ctr ISARC, Varanasi 221106, Uttar Pradesh, India

[3] Int Rice Res Inst IRRI, South Asia Hub SAH, Patancheru 502324, Telangana, India

[4] Bihar Agr Univ, Bhagalpur Rd, Sabour 813210, Bihar, India

[5] Prof Jayashankar Telangana State Agr Univ PJTSAU, Hyderabad 500030, Telangana, India

[6] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Telangana, India

来源：

GENOMICS | 2022年 / 114卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Abiotic; Biotic; Direct seeded rice; Genomics-assisted breeding; Grain yield; KASP marker; Whole genome resequencing; BACTERIAL-BLIGHT RESISTANCE; SINGLE NUCLEOTIDE POLYMORPHISMS; DISEASE RESISTANCE; GELATINIZATION TEMPERATURE; DNA MARKERS; SNP ARRAY; LEAF-AREA; GENE; SELECTION; BLAST;

D O I：

10.1016/j.ygeno.2022.110269

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The development and utilization of molecular-markers play an important role in genomics-assisted breeding during pyramiding of valuable genes. The aim of present study was to develop and validate a novel core-set of KASP (Kompetitive Allele-Specific PCR) markers associated with traits improving rice grain yield and adaptability under direct-seeded cultivation conditions. The 110 phenotypically validated KASP assays out of 171 designed KASP, include assays for biotic-resistance genes, anaerobic germination, root-traits, grain yield, lodging resistance and early-uniform emergence. The KASP assays were validated for their robustness and reliability at five different levels using diverse germplasm, segregating and advanced population, comparison with SSR markers and on F1s. The present research work will provide (i) breeding material in form of anticipated pre direct-seeded adapted rice varieties (ii) single improved breeding line with many useful genes and (iii) KASP assay information for the useful QTL/genes providing grain yield and adaptability to rice under direct-seeded cultivation conditions.

引用

页数：18

共 81 条

[1]

Ananda A., 2018, Rice Research for enhancing productivity, profitability and climate resilience, P73

[2] QTLs associated with tolerance of flooding during germination in rice (Oryza sativa L.) [J].