Effect of dilution on high-density lipoprotein associated paraoxonase-1 activity

被引：4
|
作者：
Mackness, Mike [1 ]
Mackness, Bharti [1 ]
机构：
[1] PONTECH ART, Tarragona 43840, Spain
来源：
CLINICAL BIOCHEMISTRY | 2011年 / 44卷 / 14-15期
关键词：
Paraoxonase-1; HDL; Activity assays;
D O I：
10.1016/j.clinbiochem.2011.08.002
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: To investigate the effects of dilution on high-density lipoprotein (HDL) associated paraoxonase-1 (PON1) activity. Design and methods: HDL was prepared by ultracentrifugation, diluted with TRIS buffer pH 8.2 (0-128 fold) and assayed spectrophotometrically for PON1 activity using paraoxon and phenyl acetate. Results: HDL-PON1 activity increased progressively with dilution to over 200% of the undiluted activity by 128 fold dilution (P<0.001). Conversely HDL size decreased. Conclusion: These results indicate the urgent need to standardise conditions for measuring PON1 activity. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：1270 / 1271
页数：2
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