Counter-current chromatography melamine-modified column and its separation mechanism

被引：2

作者：

Quan, Kai-Jun ^{[1
,2
,3
]}

Huang, Xin-Yi ^{[1
,2
]}

Gong, Yuan ^{[1
,2
,3
]}

Pei, Dong ^{[1
,2
]}

Duan, Wen-Da ^{[1
,2
,3
]}

Di, Duo-Long ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou, Gansu, Peoples R China

[2] Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Gansu, Peoples R China

[3] Univ Chinese Acad Sci, Beijing, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2019年 / 42卷 / 02期

基金：

国家重点研发计划;

关键词：

countercurrent chromatography; hydrogen-bond interactions; stationary phases; stationary phase retention; PARTITION CHROMATOGRAPHY; PURIFICATION;

D O I：

10.1002/jssc.201800914

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, to further verify and develop the novel counter-current chromatography modified column separate mode, a melamine modified counter-current chromatography column was prepared. Meanwhile, the modified counter-current chromatography column was used to separate stevioside and rebaudioside A with the same partition coefficient in chosen solvent system to evaluate its separation efficiency. The results show that because of the presence of intermolecular forces between melamine and model compounds, better separation could be achieved on the modified column while it's almost impossible to be separated on the unmodified column. So the results of this research further show that column modified method is a possible approach to further increase the separation ability of counter-current chromatography. Take advantage of large sample handing capacity of counter-current chromatography, the mothed may have great potential to be an efficient method of separation and preparation enantiomer compounds.

引用

页码：547 / 555

页数：9

共 24 条

[1] Hyphenating Centrifugal Partition Chromatography with Nuclear Magnetic Resonance through Automated Solid Phase Extraction [J].