Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori

被引:254
|
作者
Xia, Qingyou [2 ,3 ]
Cheng, Daojun [2 ,3 ]
Duan, Jun [2 ,3 ]
Wang, Genhong [2 ,3 ]
Cheng, Tingcai [2 ,3 ]
Zha, Xingfu [2 ,3 ]
Liu, Chun [2 ,3 ]
Zhao, Ping [2 ,3 ]
Dai, Fangyin [2 ]
Zhang, Ze [2 ,3 ]
He, Ningjia [2 ,3 ]
Zhang, Liang [1 ]
Xiang, Zhonghuai [2 ,3 ]
机构
[1] Natl Engn Ctr Beijing Biochip Technol, Beijing 102206, Peoples R China
[2] SW Univ, Key Sericultural Lab, Minist Agr, Coll Biotechnol, Chongqing 400715, Peoples R China
[3] SW Univ, Key Lab Sericultural Sci & Genom, Minist Educ, Coll Biotechnol, Chongqing 400715, Peoples R China
关键词
D O I
10.1186/gb-2007-8-8-r162
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We designed and constructed a genome-wide microarray with 22,987 70-mer oligonucleotides covering the presently known and predicted genes in the silkworm genome, and surveyed the gene expression in multiple silkworm tissues on day 3 of the fifth instar. Clusters of tissue-prevalent and tissue-specific genes and genes that are differentially expressed in different tissues were identified, and they reflect well major tissue-specific functions on the molecular level. The data presented in this study provide a new resource for annotating the silkworm genome.
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页数:13
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