Improved Preparation Method of Polyethylenimine-Hydroxyapatite and Its Chromatographic Properties

被引：4

作者：

Murakami, Yukiko ^{[1
]}

Sugo, Ken ^{[1
]}

Hirano, Masahiro ^{[1
]}

Okuyama, Tsuneo ^{[2
]}

机构：

[1] HOYA Corp, Dept Res & Dev, PENTAX New Ceram Div, Akishima, Tokyo, Japan

[2] Prot Technos Inst, Atsugi, Kanagawa, Japan

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2011年 / 46卷 / 08期

关键词：

chromatography; hydroxyapatite; nucleotide; phosphorylated protein; polyethylenimine; PERFORMANCE LIQUID-CHROMATOGRAPHY; ANION-EXCHANGE CHROMATOGRAPHY; COLUMN CHROMATOGRAPHY; PROTEINS; OLIGONUCLEOTIDES; SILICA; NUCLEOTIDES; SEPARATION;

D O I：

10.1080/01496395.2011.554950

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Previously, we developed polyethylenimine-coated hydroxyapatite as a chromatography medium. This report describes a simple, safe, productive, and improved preparation method and reports further chromatographic properties. The improved polyethylenimine-coated hydroxyapatite was prepared by use of a rotary evaporator with neither decantation, cross-linking agent, nor organic solvent. In chromatography, ovalbumin (a phosphoprotein) was separated from other proteins. Furthermore, ovalbumin derivatives or nucleotides could be separated on the basis of the number of phosphate residues. This method yielded high-quality and -quantity polyethylenimine-coated hydroxyapatite.

引用

页码：1306 / 1312

页数：7