In vitro assessment of antimicrobial, antibiofilm and larvicidal activities of bioactive nickel metal organic framework

Multidrug resistant bacteria and mosquito vectors have developed global health threat causing devastating diseases. Novel materials with potent antimicrobial and larvicidal strategies are of urgent need for eradication of disease causing pathogens. The present study focuses on simple fabrication of nickel metal organic framework (Ni-MOFs) with multifunctional biological activities. The synthesized Ni-MOFs particles were characterized by XRD, FTIR, SEM and TEM analysis. The average size of the Ni-MOF was observed to be 761 nm. The experimental results revealed that Ni-MOFs exhibited potent antibacterial activity against extended spectrum beta lactamase strain and Pseudomonas aeruginosa. Antibiofilm studies revealed that Ni-MOF exhibited dose dependant inhibition of biofilm formation against MRSA strains ATCC 33591 and clinical strain N7 with IC50 value of 15.19 +/- 1.41; 25.14 +/- 0.75 mu g/ml respectively. Further, larvicidal studies against viral vector Aedes aegypti illustrated that Ni-MOF exhibited excellent larvicidal activity in concentration dependant manner with LC50 value of 43.62 +/- 1.25 mu g/ml and LC90 value of 74.72 +/- 1.97 mu g/ml respectively. Safety evaluation study using in vivo model Anemia sauna indicated that Ni-MOFs was observed to be non-toxic toxic at lower doses, while at highest dose it exhibits toxicity with LC50 value of 138.33 +/- 3.72 mu g/ml. The biocompatible nature of Ni-MOFs depicts it as promising nanocarrier for the control of bacterial infection and mosquito population through ecofriendly approach.